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Optics Letters

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  • Editor: Alan E. Willner
  • Vol. 38, Iss. 11 — Jun. 1, 2013
  • pp: 1860–1862

Auto-aligning stimulated emission depletion microscope using adaptive optics

Travis J. Gould, Emil B. Kromann, Daniel Burke, Martin J. Booth, and Joerg Bewersdorf  »View Author Affiliations


Optics Letters, Vol. 38, Issue 11, pp. 1860-1862 (2013)
http://dx.doi.org/10.1364/OL.38.001860


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Abstract

Stimulated emission depletion (STED) microscopy provides diffraction-unlimited resolution in fluorescence microscopy. Imaging at the nanoscale, however, requires precise alignment of the depletion and excitation laser foci of the STED microscope. We demonstrate here that adaptive optics can be implemented to automatically align STED and confocal images with a precision of 4.3±2.3nm.

© 2013 Optical Society of America

OCIS Codes
(100.6640) Image processing : Superresolution
(110.0180) Imaging systems : Microscopy
(180.2520) Microscopy : Fluorescence microscopy
(070.6120) Fourier optics and signal processing : Spatial light modulators

ToC Category:
Microscopy

History
Original Manuscript: March 22, 2013
Manuscript Accepted: April 17, 2013
Published: May 22, 2013

Virtual Issues
Vol. 8, Iss. 7 Virtual Journal for Biomedical Optics

Citation
Travis J. Gould, Emil B. Kromann, Daniel Burke, Martin J. Booth, and Joerg Bewersdorf, "Auto-aligning stimulated emission depletion microscope using adaptive optics," Opt. Lett. 38, 1860-1862 (2013)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-38-11-1860


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References

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