We experimentally demonstrate an all-diffractive optical setup for digital lensless holographic microscopy with easy wavelength line selection and micrometric resolution. In the proposed system, an ultrashort laser pulse is focused with a diffractive lens (DL) onto a pinhole of diameter close to its central wavelength to achieve a highly spatially coherent illumination cone as well as a spectral line with narrow width. To scan the complete spectrum of the light source the DL is displaced with respect to the pinhole plane. The proposed microscopy setup allows us to spectrally separate contributions from different sections of a sample, which may be attractive for several applications in life sciences.
© 2013 Optical Society of America
Original Manuscript: April 26, 2013
Manuscript Accepted: May 15, 2013
Published: June 11, 2013
Vol. 8, Iss. 7 Virtual Journal for Biomedical Optics
Omel Mendoza-Yero, Enrique Tajahuerce, Jesús Lancis, and Jorge Garcia-Sucerquia, "Diffractive digital lensless holographic microscopy with fine spectral tuning," Opt. Lett. 38, 2107-2109 (2013)