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Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 38, Iss. 14 — Jul. 15, 2013
  • pp: 2401–2403

Correction of cell-induced optical aberrations in a fluorescence fluctuation microscope

Charles-Edouard Leroux, Alexei Grichine, Irène Wang, and Antoine Delon  »View Author Affiliations

Optics Letters, Vol. 38, Issue 14, pp. 2401-2403 (2013)

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We describe the effect of optical aberrations on fluorescence fluctuations microscopy (FFM), when focusing through a single living cell. FFM measurements are performed in an aqueous fluorescent solution and prove to be a highly sensitive tool to assess the optical aberrations introduced by the cell. We demonstrate an adaptive optics (AO) system to remove the aberration-related bias in the FFM measurements. Our data show that AO is not only useful when imaging deep in tissues but also when performing FFM measurements through a single cellular layer. This work paves the way for the application of FFM to complex three-dimensional multicellular samples.

© 2013 Optical Society of America

OCIS Codes
(010.1080) Atmospheric and oceanic optics : Active or adaptive optics
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(180.1790) Microscopy : Confocal microscopy

ToC Category:

Original Manuscript: April 24, 2013
Revised Manuscript: June 6, 2013
Manuscript Accepted: June 6, 2013
Published: July 5, 2013

Virtual Issues
Vol. 8, Iss. 8 Virtual Journal for Biomedical Optics

Charles-Edouard Leroux, Alexei Grichine, Irène Wang, and Antoine Delon, "Correction of cell-induced optical aberrations in a fluorescence fluctuation microscope," Opt. Lett. 38, 2401-2403 (2013)

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