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Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 38, Iss. 14 — Jul. 15, 2013
  • pp: 2592–2595

Optical clearing-aided photoacoustic microscopy with enhanced resolution and imaging depth

Yong Zhou, Junjie Yao, and Lihong V. Wang  »View Author Affiliations

Optics Letters, Vol. 38, Issue 14, pp. 2592-2595 (2013)

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Due to strong light scattering in tissue, both the spatial resolution and maximum penetration depth of optical-resolution photoacoustic microscopy (OR-PAM) deteriorate sharply with depth. To reduce tissue scattering, we propose to use glycerol as an optical clearing agent in OR-PAM. Our results show that the imaging performance of OR-PAM can be greatly enhanced by optical clearing both in vitro and in vivo.

© 2013 Optical Society of America

OCIS Codes
(170.3660) Medical optics and biotechnology : Light propagation in tissues
(170.5120) Medical optics and biotechnology : Photoacoustic imaging
(170.6930) Medical optics and biotechnology : Tissue
(110.0113) Imaging systems : Imaging through turbid media

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: June 3, 2013
Manuscript Accepted: June 13, 2013
Published: July 15, 2013

Virtual Issues
Vol. 8, Iss. 8 Virtual Journal for Biomedical Optics

Yong Zhou, Junjie Yao, and Lihong V. Wang, "Optical clearing-aided photoacoustic microscopy with enhanced resolution and imaging depth," Opt. Lett. 38, 2592-2595 (2013)

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