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Optics Letters

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  • Editor: Alan E. Willner
  • Vol. 38, Iss. 15 — Aug. 1, 2013
  • pp: 2711–2713

Multifocus optical-resolution photoacoustic microscopy using stimulated Raman scattering and chromatic aberration

Parsin Hajireza, Alexander Forbrich, and Roger J. Zemp  »View Author Affiliations


Optics Letters, Vol. 38, Issue 15, pp. 2711-2713 (2013)
http://dx.doi.org/10.1364/OL.38.002711


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Abstract

In this Letter, multifocus optical-resolution photoacoustic microscopy is demonstrated using wavelength tuning and chromatic aberration for depth scanning. Discrete focal zones at several depth locations were created by refocusing light from a polarization-maintaining single-mode fiber pumped by a nanosecond fiber laser. The fiber and laser parameters were chosen to take advantage of stimulated Raman scattering (SRS) in the fiber to create a multiwavelength output that could then be bandpass filtered. The collimator lens and objective lens are chosen to take advantage of chromatic aberration in which each generated SRS wavelength peak focuses at a slightly different depth. The maximum amplitude of photoacoustic signals is mapped to form C-scan images. Additionally, all wavelength peaks fired simultaneously offers improved depth-of-field structural imaging at the cost of slight degradation of mainlobe-to-sidelobe ratios. Wavelength-tuned depth scanning over more than 440 μm is demonstrated, significantly greater than the 100μm depth of field predicted from our focused Gaussian beams. The improved depth of focus could be valuable for structural imaging of microvascular morphology without the need for mechanical scanning in the depth direction.

© 2013 Optical Society of America

OCIS Codes
(110.0110) Imaging systems : Imaging systems
(110.0180) Imaging systems : Microscopy
(110.5120) Imaging systems : Photoacoustic imaging
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.5120) Medical optics and biotechnology : Photoacoustic imaging
(290.5860) Scattering : Scattering, Raman

ToC Category:
Imaging Systems

History
Original Manuscript: May 23, 2013
Revised Manuscript: June 19, 2013
Manuscript Accepted: June 25, 2013
Published: July 23, 2013

Virtual Issues
Vol. 8, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Parsin Hajireza, Alexander Forbrich, and Roger J. Zemp, "Multifocus optical-resolution photoacoustic microscopy using stimulated Raman scattering and chromatic aberration," Opt. Lett. 38, 2711-2713 (2013)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-38-15-2711


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References

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