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Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 38, Iss. 15 — Aug. 1, 2013
  • pp: 2786–2788

System and methods for wide-field quantitative fluorescence imaging during neurosurgery

Pablo A. Valdes, Valerie L. Jacobs, Brian C. Wilson, Frederic Leblond, David W. Roberts, and Keith D. Paulsen  »View Author Affiliations

Optics Letters, Vol. 38, Issue 15, pp. 2786-2788 (2013)

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We report an accurate, precise and sensitive method and system for quantitative fluorescence image-guided neurosurgery. With a low-noise, high-dynamic-range CMOS array, we perform rapid (integration times as low as 50 ms per wavelength) hyperspectral fluorescence and diffuse reflectance detection and apply a correction algorithm to compensate for the distorting effects of tissue absorption and scattering. Using this approach, we generated quantitative wide-field images of fluorescence in tissue-simulating phantoms for the fluorophore PpIX, having concentrations and optical absorption and scattering variations over clinically relevant ranges. The imaging system was tested in a rodent model of glioma, detecting quantitative levels down to 20ng/ml. The resulting performance is a significant advance on existing wide-field quantitative imaging techniques, and provides performance comparable to a point-spectroscopy probe that has previously demonstrated significant potential for improved detection of malignant brain tumors during surgical resection.

© 2013 Optical Society of America

OCIS Codes
(170.1610) Medical optics and biotechnology : Clinical applications
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.3660) Medical optics and biotechnology : Light propagation in tissues
(170.6935) Medical optics and biotechnology : Tissue characterization

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: April 15, 2013
Revised Manuscript: June 3, 2013
Manuscript Accepted: June 25, 2013
Published: July 29, 2013

Virtual Issues
Vol. 8, Iss. 9 Virtual Journal for Biomedical Optics

Pablo A. Valdes, Valerie L. Jacobs, Brian C. Wilson, Frederic Leblond, David W. Roberts, and Keith D. Paulsen, "System and methods for wide-field quantitative fluorescence imaging during neurosurgery," Opt. Lett. 38, 2786-2788 (2013)

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