Abstract
We report an accurate, precise and sensitive method and system for quantitative fluorescence image-guided neurosurgery. With a low-noise, high-dynamic-range CMOS array, we perform rapid (integration times as low as 50 ms per wavelength) hyperspectral fluorescence and diffuse reflectance detection and apply a correction algorithm to compensate for the distorting effects of tissue absorption and scattering. Using this approach, we generated quantitative wide-field images of fluorescence in tissue-simulating phantoms for the fluorophore PpIX, having concentrations and optical absorption and scattering variations over clinically relevant ranges. The imaging system was tested in a rodent model of glioma, detecting quantitative levels down to . The resulting performance is a significant advance on existing wide-field quantitative imaging techniques, and provides performance comparable to a point-spectroscopy probe that has previously demonstrated significant potential for improved detection of malignant brain tumors during surgical resection.
© 2013 Optical Society of America
Full Article | PDF ArticleMore Like This
Michael Jermyn, Yoann Gosselin, Pablo A. Valdes, Mira Sibai, Kolbein Kolste, Jeanne Mercier, Leticia Angulo, David W. Roberts, Keith D. Paulsen, Kevin Petrecca, Olivier Daigle, Brian C. Wilson, and Frederic Leblond
Biomed. Opt. Express 6(12) 5063-5074 (2015)
P. A. Valdés, F. Leblond, A. Kim, B. C. Wilson, K. D. Paulsen, and D. W. Roberts
Opt. Lett. 37(11) 1817-1819 (2012)
Mira Sibai, Israel Veilleux, Jonathan T. Elliott, Frederic Leblond, and Brian C. Wilson
Biomed. Opt. Express 6(12) 4923-4933 (2015)