The use of femtosecond laser radiation in digital lensless holographic microscopy (DLHM) to image biological samples is presented. A mode-locked Ti:Sa laser that emits ultrashort pulses of 12 fs intensity FWHM, with 800 nm mean wavelength, at 75 MHz repetition rate is used as a light source. For comparison purposes, the light from a light-emitting diode is also used. A section of the head of a drosophila melanogaster fly is studied with both light sources. The experimental results show very different effects of the pinhole size on the spatial resolution with DLHM. Unaware phenomena on the field of the DLHM are analyzed.
© 2013 Optical Society of America
Original Manuscript: April 29, 2013
Revised Manuscript: July 3, 2013
Manuscript Accepted: July 15, 2013
Published: August 19, 2013
Vol. 8, Iss. 10 Virtual Journal for Biomedical Optics
Omel Mendoza-Yero, Alejandro Calabuig, Enrique Tajahuerce, Jesús Lancis, Pedro Andrés, and Jorge Garcia-Sucerquia, "Femtosecond digital lensless holographic microscopy to image biological samples," Opt. Lett. 38, 3205-3207 (2013)