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Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Editor: Alan E. Willner
  • Vol. 38, Iss. 17 — Sep. 1, 2013
  • pp: 3219–3222

Doubling the resolution of spatial-light-modulator-based differential interference contrast microscopy by structured illumination

Jianling Chen, Xiaohua Lv, and Shaoqun Zeng  »View Author Affiliations


Optics Letters, Vol. 38, Issue 17, pp. 3219-3222 (2013)
http://dx.doi.org/10.1364/OL.38.003219


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Abstract

Recently developed spatial light modulator (SLM)-based differential interference contrast (DIC) microscopy [Opt. Lett. 34, 2988 (2009)] reveals flexibility on the implementation of DIC imaging. However, its numerical aperture (spatial resolution) is limited to maintain sufficient interference contrast, because it requires two beams to interfere. We present a structured illumination (SI) SLM-based DIC microscopy to effectively improve the lateral resolution of the SLM-based DIC microscopy. The SI field is generated and controlled by an adjustable grating displayed on an SLM. The SI SLM-based DIC expands the bandwidth of the coherent transfer function of the SLM-based DIC imaging system, thus improving the spatial resolution. The reconstructed SI SLM-based DIC image exhibits lateral resolution of approximately 208 nm, doubling that of the common SLM-based DIC image (approximately 415 nm). SI SLM-based DIC microscopy has the potential for achieving high-resolution quantitative phase images.

© 2013 Optical Society of America

OCIS Codes
(100.6640) Image processing : Superresolution
(110.1650) Imaging systems : Coherence imaging
(180.3170) Microscopy : Interference microscopy

ToC Category:
Microscopy

History
Original Manuscript: July 5, 2013
Manuscript Accepted: July 22, 2013
Published: August 19, 2013

Virtual Issues
Vol. 8, Iss. 10 Virtual Journal for Biomedical Optics

Citation
Jianling Chen, Xiaohua Lv, and Shaoqun Zeng, "Doubling the resolution of spatial-light-modulator-based differential interference contrast microscopy by structured illumination," Opt. Lett. 38, 3219-3222 (2013)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-38-17-3219

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