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Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 38, Iss. 17 — Sep. 1, 2013
  • pp: 3249–3252

Pulsed-light imaging for fluorescence guided surgery under normal room lighting

Kristian Sexton, Scott C. Davis, David McClatchy, III, Pablo A. Valdes, Stephen C. Kanick, Keith D. Paulsen, David W. Roberts, and Brian W. Pogue  »View Author Affiliations

Optics Letters, Vol. 38, Issue 17, pp. 3249-3252 (2013)

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Fluorescence guided surgery (FGS) is an emerging technology that has demonstrated improved surgical outcomes. However, dim lighting conditions required by current FGS systems are disruptive to standard surgical workflow. We present a novel FGS system capable of imaging fluorescence under normal room light by using pulsed excitation and gated acquisition. Images from tissue-simulating phantoms confirm visual detection down to 0.25 μM of protoporphyrin IX under 125 μW / cm 2 of ambient light, more than an order of magnitude lower than that measured with the Zeiss Pentero in the dark. Resection of orthotopic brain tumors in mice also suggests that the pulsed-light system provides superior sensitivity in vivo.

© 2013 Optical Society of America

OCIS Codes
(110.0110) Imaging systems : Imaging systems
(110.2970) Imaging systems : Image detection systems
(170.1610) Medical optics and biotechnology : Clinical applications
(170.3890) Medical optics and biotechnology : Medical optics instrumentation
(230.3670) Optical devices : Light-emitting diodes

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: May 3, 2013
Revised Manuscript: June 28, 2013
Manuscript Accepted: July 8, 2013
Published: August 20, 2013

Virtual Issues
Vol. 8, Iss. 10 Virtual Journal for Biomedical Optics

Kristian Sexton, Scott C. Davis, David McClatchy, Pablo A. Valdes, Stephen C. Kanick, Keith D. Paulsen, David W. Roberts, and Brian W. Pogue, "Pulsed-light imaging for fluorescence guided surgery under normal room lighting," Opt. Lett. 38, 3249-3252 (2013)

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