We present theoretical and experimental results of axial displacement of objects relative to a fixed condenser focal plane (FP) in optical projection tomographic microscopy (OPTM). OPTM produces three-dimensional, reconstructed images of single cells from two-dimensional projections. The cell rotates in a microcapillary to acquire projections from different perspectives where the objective FP is scanned through the cell while the condenser FP remains fixed at the center of the microcapillary. This work uses a combination of experimental and theoretical methods to improve the OPTM instrument design.
© 2013 Optical Society of America
Original Manuscript: May 8, 2013
Revised Manuscript: August 5, 2013
Manuscript Accepted: August 5, 2013
Published: August 26, 2013
Vol. 8, Iss. 10 Virtual Journal for Biomedical Optics
Ryan L. Coe and Eric J. Seibel, "Experimental and theoretical analysis for improved microscope design of optical projection tomographic microscopy," Opt. Lett. 38, 3398-3401 (2013)