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Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 38, Iss. 18 — Sep. 15, 2013
  • pp: 3538–3541

Noninterferometric single-shot quantitative phase microscopy

Chao Zuo, Qian Chen, Weijuan Qu, and Anand Asundi  »View Author Affiliations

Optics Letters, Vol. 38, Issue 18, pp. 3538-3541 (2013)

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We present a noninterferometric single-shot quantitative phase microscopy technique with the use of the transport of intensity equation (TIE). The optical configuration is based on a Michelson-like architecture attached to a nonmodified inverted transmission bright field microscope. Two laterally separated images from different focal planes can be obtained simultaneously by a single camera exposure, enabling the TIE phase recovery to be performed at frame rates that are only camera limited. Precise measurement of a microlens array validates the principle and demonstrates the accuracy of the method. Investigations of chemical-induced apoptosis and the phagocytosis process of macrophages are then presented, suggesting that the method developed can provide promising applications in the dynamic study of cellular processes.

© 2013 Optical Society of America

OCIS Codes
(100.3010) Image processing : Image reconstruction techniques
(100.5070) Image processing : Phase retrieval
(110.0180) Imaging systems : Microscopy
(110.6880) Imaging systems : Three-dimensional image acquisition
(180.0180) Microscopy : Microscopy

ToC Category:
Imaging Systems

Original Manuscript: July 15, 2013
Revised Manuscript: August 10, 2013
Manuscript Accepted: August 10, 2013
Published: September 5, 2013

Virtual Issues
Vol. 8, Iss. 10 Virtual Journal for Biomedical Optics

Chao Zuo, Qian Chen, Weijuan Qu, and Anand Asundi, "Noninterferometric single-shot quantitative phase microscopy," Opt. Lett. 38, 3538-3541 (2013)

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