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Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Editor: Alan E. Willner
  • Vol. 38, Iss. 19 — Oct. 1, 2013
  • pp: 3712–3715

Approach to multiparticle parallel tracking in thick samples with three-dimensional nanoresolution

Danni Chen, Bin Yu, Heng Li, Yingdong Huo, Bo Cao, Gaixia Xu, and Hanben Niu  »View Author Affiliations


Optics Letters, Vol. 38, Issue 19, pp. 3712-3715 (2013)
http://dx.doi.org/10.1364/OL.38.003712


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Abstract

This Letter proposes a method referred to as distorted grating (DG) and double-helix point spread function (DH-PSF) combination microscopy (DDCM), which is capable of multiparticle parallel localization and tracking in a transparent sample thicker than 10 μm, the thickness of cells. A special phase mask, combining the field depth extension capabilities of DG with the three-dimensional (3D) nanolocalization capabilities of the DH-PSF, is designed for multiparticle parallel localization. Time-lapse tracking of one particle moving along the z axis and parallel tracking of two particles are simulated. Results demonstrate that, with only a single snapshot, particles can be localized, tracking with 3D nanoresolution wherever they are. The theoretical localization precisions of DDCM, DH-PSF, and multifocus microscopy are compared. DDCM results in almost constant localization precisions in all three dimensions for a depth of field larger than 10 μm. DDCM is expected to become a tool in investigations of important dynamic events in living cells.

© 2013 Optical Society of America

OCIS Codes
(100.6640) Image processing : Superresolution
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(100.4999) Image processing : Pattern recognition, target tracking

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: July 10, 2013
Revised Manuscript: August 16, 2013
Manuscript Accepted: August 19, 2013
Published: September 16, 2013

Citation
Danni Chen, Bin Yu, Heng Li, Yingdong Huo, Bo Cao, Gaixia Xu, and Hanben Niu, "Approach to multiparticle parallel tracking in thick samples with three-dimensional nanoresolution," Opt. Lett. 38, 3712-3715 (2013)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-38-19-3712

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