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Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 38, Iss. 19 — Oct. 1, 2013
  • pp: 3712–3715

Approach to multiparticle parallel tracking in thick samples with three-dimensional nanoresolution

Danni Chen, Bin Yu, Heng Li, Yingdong Huo, Bo Cao, Gaixia Xu, and Hanben Niu  »View Author Affiliations

Optics Letters, Vol. 38, Issue 19, pp. 3712-3715 (2013)

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This Letter proposes a method referred to as distorted grating (DG) and double-helix point spread function (DH-PSF) combination microscopy (DDCM), which is capable of multiparticle parallel localization and tracking in a transparent sample thicker than 10 μm, the thickness of cells. A special phase mask, combining the field depth extension capabilities of DG with the three-dimensional (3D) nanolocalization capabilities of the DH-PSF, is designed for multiparticle parallel localization. Time-lapse tracking of one particle moving along the z axis and parallel tracking of two particles are simulated. Results demonstrate that, with only a single snapshot, particles can be localized, tracking with 3D nanoresolution wherever they are. The theoretical localization precisions of DDCM, DH-PSF, and multifocus microscopy are compared. DDCM results in almost constant localization precisions in all three dimensions for a depth of field larger than 10 μm. DDCM is expected to become a tool in investigations of important dynamic events in living cells.

© 2013 Optical Society of America

OCIS Codes
(100.6640) Image processing : Superresolution
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(100.4999) Image processing : Pattern recognition, target tracking

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: July 10, 2013
Revised Manuscript: August 16, 2013
Manuscript Accepted: August 19, 2013
Published: September 16, 2013

Danni Chen, Bin Yu, Heng Li, Yingdong Huo, Bo Cao, Gaixia Xu, and Hanben Niu, "Approach to multiparticle parallel tracking in thick samples with three-dimensional nanoresolution," Opt. Lett. 38, 3712-3715 (2013)

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  1. A. Sergé, N. Bertaux, H. Rigneault, and D. Marguet, Nat. Methods 5, 687 (2008). [CrossRef]
  2. I. Mellman, Annu. Rev. Cell Dev. Biol. 12, 575 (1996). [CrossRef]
  3. F. R. Maxfield and T. E. McGraw, Nat. Rev. Mol. Cell Biol. 5, 121 (2004). [CrossRef]
  4. W. E. Moerner and D. P. Fromm, Rev. Sci. Instrum. 74, 3597 (2003). [CrossRef]
  5. A. Yildiz, J. N. Forkey, S. A. McKinney, T. Ha, Y. E. Goldman, and P. R. Selvin, Science 300, 2061 (2003). [CrossRef]
  6. B. Huang, W. Wang, M. Bates, and X. Zhuang, Science 319, 810 (2008). [CrossRef]
  7. S. Ram, P. Prabhat, E. S. Ward, and R. J. Ober, Opt. Express 17, 6881 (2009). [CrossRef]
  8. M. F. Juette, T. J. Gould, M. D. Lessard, M. J. Mlodzianoski, B. S. Nagpure, B. T. Bennett, S. T. Hess, and J. Bewersdorf, Nat. Methods 5, 527 (2008). [CrossRef]
  9. T. Erdal, B. Hamza, H. B. Benjamin, and R. S. Paul, Nano Lett. 7, 2043 (2007). [CrossRef]
  10. G. Shtengel, J. A. Galbraith, C. G. Galbraith, J. Lippincott-Schwartz, J. M. Gillette, S. Manley, R. Sougrat, C. M. Waterman, P. Kanchanawong, M. W. Davidson, R. D. Fetter, and H. F. Hess, Proc. Natl. Acad. Sci. USA 106, 3125 (2009). [CrossRef]
  11. S. R. P. Pavani, M. A. Thompson, J. S. Biteen, S. J. Lord, N. Liu, R. J. Twieg, R. Piestun, and W. E. Moerner, Proc. Natl. Acad. Sci. USA 106, 2995 (2009). [CrossRef]
  12. S. R. P. Pavani and R. Piestun, Opt. Express 16, 22048 (2008). [CrossRef]
  13. M. D. Lew, S. F. Lee, M. Badieirostami, and W. E. Moerner, Opt. Lett. 36, 202 (2011). [CrossRef]
  14. P. Prabhat, S. Ram, E. S. Ward, and R. J. Ober, IEEE Trans. Nanobiosci. 3, 237 (2004). [CrossRef]
  15. P. Prabhat, S. Ram, E. S. Ward, and R. J. Ober, Proc. SPIE 6090, 60900L (2006). [CrossRef]
  16. S. Ram, D. Kim, E. S. Ward, and R. J. Ober, Biophys. J. 103, 1594 (2012). [CrossRef]
  17. S. Abrahamsson, J. Chen, B. Hajj, S. Stallinga, A. Y. Katsov, J. Wisniewski, G. Mizuguchi, P. Soule, F. Mueller, C. D. Darzacq, X. Darzacq, C. Wu, C. I. Bargmann, D. A. Agard, M. Dahan, and M. G. L. Gustafsson, Nat. Methods 10, 60 (2012). [CrossRef]
  18. P. M. Blanchard and A. H. Greenaway, Appl. Opt. 38, 6692 (1999). [CrossRef]
  19. R. Piestun, Y. Y. Schechner, and J. Shamir, J. Opt. Soc. Am. A 17, 294 (2000). [CrossRef]
  20. S. R. P. Pavani and R. Piestun, Opt. Express 16, 3484 (2008). [CrossRef]
  21. R. J. Ober, S. Ram, and E. S. Ward, Biophys. J. 86, 1185 (2004). [CrossRef]
  22. M. J. Rust, M. Bates, and X. Zhuang, Nat. Methods 3, 793 (2006). [CrossRef]
  23. E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, Science 313, 1642 (2006). [CrossRef]
  24. S. T. Hess, T. P. K. Girirajan, and M. D. Mason, Biophys. J. 91, 4258 (2006). [CrossRef]
  25. G. Grover, S. Quirin, C. Fiedler, and R. Piestun, Biomed. Opt. Express 2, 3010 (2011). [CrossRef]
  26. P. A. Dalgarno, H. I. C. Dalgarno, A. Putoud, R. Lambert, L. Paterson, D. C. Logan, D. P. Towers, R. J. Warburton, and A. H. Greenaway, Opt. Express 18, 877 (2010). [CrossRef]
  27. C. Wu, T. Schneider, M. Zeigler, J. Yu, P. Schiro, D. Burnham, J. D. McNeill, and D. T. Chiu, J. Am. Chem. Soc. 132, 15410 (2010). [CrossRef]
  28. J. C. Vaughan, S. Jia, and X. Zhuang, Nat. Methods 9, 1181 (2012). [CrossRef]

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