OSA's Digital Library

Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Editor: Alan E. Willner
  • Vol. 38, Iss. 2 — Jan. 15, 2013
  • pp: 145–147

Multicolor stimulated Raman scattering microscopy with a rapidly tunable optical parametric oscillator

Lingjie Kong, Minbiao Ji, Gary R. Holtom, Dan Fu, Christian W. Freudiger, and X. Sunney Xie  »View Author Affiliations


Optics Letters, Vol. 38, Issue 2, pp. 145-147 (2013)
http://dx.doi.org/10.1364/OL.38.000145


View Full Text Article

Enhanced HTML    Acrobat PDF (260 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

Stimulated Raman scattering (SRS) microscopy allows label-free chemical imaging based on vibrational spectroscopy. Narrowband excitation with picosecond lasers creates the highest signal levels and enables imaging speeds up to video-rate, but it sacrifices chemical specificity in samples with overlapping bands compared to broadband (multiplex) excitation. We develop a rapidly tunable picosecond optical parametric oscillator with an electro-optical tunable Lyot filter, and demonstrate multicolor SRS microscopy with synchronized line-by-line wavelength tuning to avoid spectral artifacts due to sample movement. We show sensitive imaging of three different kinds of polymer beads and live HeLa cells with moving intracellular lipid droplets.

© 2013 Optical Society of America

OCIS Codes
(190.4970) Nonlinear optics : Parametric oscillators and amplifiers
(180.4315) Microscopy : Nonlinear microscopy
(180.5655) Microscopy : Raman microscopy

ToC Category:
Microscopy

History
Original Manuscript: November 8, 2012
Manuscript Accepted: November 30, 2012
Published: January 8, 2013

Virtual Issues
Vol. 8, Iss. 2 Virtual Journal for Biomedical Optics

Citation
Lingjie Kong, Minbiao Ji, Gary R. Holtom, Dan Fu, Christian W. Freudiger, and X. Sunney Xie, "Multicolor stimulated Raman scattering microscopy with a rapidly tunable optical parametric oscillator," Opt. Lett. 38, 145-147 (2013)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-38-2-145

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited