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Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 38, Iss. 21 — Nov. 1, 2013
  • pp: 4510–4513

Resolution and contrast enhancement in coherent anti-Stokes Raman-scattering microscopy

Alicja Gasecka, Amy Daradich, Harold Dehez, Michel Piché, and Daniel Côté  »View Author Affiliations

Optics Letters, Vol. 38, Issue 21, pp. 4510-4513 (2013)

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We implement switching laser mode coherent anti-Stokes Raman-scattering (SLAM-CARS) microscopy to enhance the spatial resolution and contrast in label-free vibrational microscopy. The method, based on the intensity difference between two images obtained with Gaussian and doughnut-shaped modes, does not depend on the specimen and relies on minimal modifications of the typical CARS setup. We demonstrate subdiffraction resolution imaging of myelin sheaths in a mouse brainstem. A lateral resolution of 0.36λp is achieved.

© 2013 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(170.0110) Medical optics and biotechnology : Imaging systems
(190.4380) Nonlinear optics : Nonlinear optics, four-wave mixing
(180.5655) Microscopy : Raman microscopy

ToC Category:

Original Manuscript: August 22, 2013
Revised Manuscript: October 4, 2013
Manuscript Accepted: October 4, 2013
Published: October 31, 2013

Virtual Issues
Vol. 9, Iss. 1 Virtual Journal for Biomedical Optics

Alicja Gasecka, Amy Daradich, Harold Dehez, Michel Piché, and Daniel Côté, "Resolution and contrast enhancement in coherent anti-Stokes Raman-scattering microscopy," Opt. Lett. 38, 4510-4513 (2013)

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