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Optics Letters

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  • Editor: Alan E. Willner
  • Vol. 38, Iss. 21 — Nov. 1, 2013
  • pp: 4510–4513

Resolution and contrast enhancement in coherent anti-Stokes Raman-scattering microscopy

Alicja Gasecka, Amy Daradich, Harold Dehez, Michel Piché, and Daniel Côté  »View Author Affiliations


Optics Letters, Vol. 38, Issue 21, pp. 4510-4513 (2013)
http://dx.doi.org/10.1364/OL.38.004510


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Abstract

We implement switching laser mode coherent anti-Stokes Raman-scattering (SLAM-CARS) microscopy to enhance the spatial resolution and contrast in label-free vibrational microscopy. The method, based on the intensity difference between two images obtained with Gaussian and doughnut-shaped modes, does not depend on the specimen and relies on minimal modifications of the typical CARS setup. We demonstrate subdiffraction resolution imaging of myelin sheaths in a mouse brainstem. A lateral resolution of 0.36λp is achieved.

© 2013 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(170.0110) Medical optics and biotechnology : Imaging systems
(190.4380) Nonlinear optics : Nonlinear optics, four-wave mixing
(180.5655) Microscopy : Raman microscopy

ToC Category:
Microscopy

History
Original Manuscript: August 22, 2013
Revised Manuscript: October 4, 2013
Manuscript Accepted: October 4, 2013
Published: October 31, 2013

Virtual Issues
Vol. 9, Iss. 1 Virtual Journal for Biomedical Optics

Citation
Alicja Gasecka, Amy Daradich, Harold Dehez, Michel Piché, and Daniel Côté, "Resolution and contrast enhancement in coherent anti-Stokes Raman-scattering microscopy," Opt. Lett. 38, 4510-4513 (2013)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-38-21-4510


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References

  1. H. Wang, Y. Fu, P. Zickmund, R. Shi, and J. X. Cheng, Biophys. J. 89, 581 (2005). [CrossRef]
  2. T. T. Le, C. W. Rehrer, T. B. Huff, M. B. Nichols, I. G. Camarillo, and J. X. Cheng, Mol. Imaging 6, 205 (2007).
  3. C. L. Evans, E. O. Potma, M. Puorishaag, D. Côté, C. P. Lin, and X. S. Xie, Proc. Natl. Acad. Sci. USA 102, 16807 (2005). [CrossRef]
  4. J. X. Cheng, A. Volkmer, and X. S. Xie, J. Opt. Soc. Am. B 19, 1363 (2002). [CrossRef]
  5. E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, Science 313, 1642 (2006). [CrossRef]
  6. M. J. Rust, M. Bates, and X. Zhuang, Nat. Methods 3, 793 (2006). [CrossRef]
  7. S. W. Hell and J. Wichmann, Opt. Lett. 19, 780 (1994). [CrossRef]
  8. S. W. Hell, Science 316, 1153 (2007). [CrossRef]
  9. H. Dehez, M. Piché, and Y. D. Koninck, Opt. Express 21, 15912 (2013). [CrossRef]
  10. C. Kuang, S. Li, W. Liu, X. Hao, Z. Gu, Y. Wang, J. Ge, H. Li, and X. Liu, Sci. Rep. 3, 1441 (2013). [CrossRef]
  11. R. Heintzmann, V. Sarafis, P. Munroe, J. Nailon, Q. S. Hanley, and T. M. Jovin, Micron 34, 293 (2003). [CrossRef]

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