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Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 38, Iss. 22 — Nov. 15, 2013
  • pp: 4526–4529

En face optical coherence tomography of transient light response at photoreceptor outer segments in living frog eyecup

Benquan Wang, Rongwen Lu, Qiuxiang Zhang, Yuqiang Jiang, and Xincheng Yao  »View Author Affiliations

Optics Letters, Vol. 38, Issue 22, pp. 4526-4529 (2013)

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This study was designed to test the feasibility of en face mapping of the transient intrinsic optical signal (IOS) response at photoreceptor outer segments and to assess the effect of spatial resolution on functional IOS imaging of retinal photoreceptors. A line-scan optical coherence tomography (LS-OCT) was constructed to achieve depth-resolved functional IOS imaging of living frog eyecups. Rapid en face OCT revealed transient IOS almost immediately (<3ms) after the onset of visible light flashes at photoreceptor outer segments. Quantitative analysis indicated that the IOS kinetics may reflect dynamics of G-protein binding and releasing in early phases of visual transduction, and high resolution is essential to differentiate positive and negative IOS changes in adjacent locations.

© 2013 Optical Society of America

OCIS Codes
(170.0110) Medical optics and biotechnology : Imaging systems
(170.2655) Medical optics and biotechnology : Functional monitoring and imaging
(170.5755) Medical optics and biotechnology : Retina scanning

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: August 22, 2013
Manuscript Accepted: September 30, 2013
Published: November 4, 2013

Virtual Issues
Vol. 9, Iss. 1 Virtual Journal for Biomedical Optics

Benquan Wang, Rongwen Lu, Qiuxiang Zhang, Yuqiang Jiang, and Xincheng Yao, "En face optical coherence tomography of transient light response at photoreceptor outer segments in living frog eyecup," Opt. Lett. 38, 4526-4529 (2013)

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