The development of DNA analysis methods is rapidly expanding as interest in characterizing subtle variations increases in biomedicine. A promising approach is based on evanescent field sensors that monitor the hybridization process in real time. However, one challenge is discriminating between nonspecific and specific attachment. Here, we demonstrate a hybridization sensor based on an integrated toroidal optical microcavity. The surface is functionalized with ssDNA using an epoxide method, and the evanescent wave of the microresonator excites a fluorescent label on the complementary ssDNA during hybridization. Based on a temporal analysis, the different binding regimes can be identified.
© 2013 Optical Society of America
Original Manuscript: July 25, 2013
Revised Manuscript: September 26, 2013
Manuscript Accepted: October 7, 2013
Published: November 11, 2013
Vol. 9, Iss. 1 Virtual Journal for Biomedical Optics
Rasheeda M. Hawk, Maria V. Chistiakova, and Andrea M. Armani, "Monitoring DNA hybridization using optical microcavities," Opt. Lett. 38, 4690-4693 (2013)