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Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Editor: Alan E. Willner
  • Vol. 38, Iss. 22 — Nov. 15, 2013
  • pp: 4690–4693

Monitoring DNA hybridization using optical microcavities

Rasheeda M. Hawk, Maria V. Chistiakova, and Andrea M. Armani  »View Author Affiliations


Optics Letters, Vol. 38, Issue 22, pp. 4690-4693 (2013)
http://dx.doi.org/10.1364/OL.38.004690


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Abstract

The development of DNA analysis methods is rapidly expanding as interest in characterizing subtle variations increases in biomedicine. A promising approach is based on evanescent field sensors that monitor the hybridization process in real time. However, one challenge is discriminating between nonspecific and specific attachment. Here, we demonstrate a hybridization sensor based on an integrated toroidal optical microcavity. The surface is functionalized with ssDNA using an epoxide method, and the evanescent wave of the microresonator excites a fluorescent label on the complementary ssDNA during hybridization. Based on a temporal analysis, the different binding regimes can be identified.

© 2013 Optical Society of America

OCIS Codes
(130.6010) Integrated optics : Sensors
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(230.5750) Optical devices : Resonators

ToC Category:
Integrated Optics

History
Original Manuscript: July 25, 2013
Revised Manuscript: September 26, 2013
Manuscript Accepted: October 7, 2013
Published: November 11, 2013

Virtual Issues
Vol. 9, Iss. 1 Virtual Journal for Biomedical Optics

Citation
Rasheeda M. Hawk, Maria V. Chistiakova, and Andrea M. Armani, "Monitoring DNA hybridization using optical microcavities," Opt. Lett. 38, 4690-4693 (2013)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-38-22-4690


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