OSA's Digital Library

Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Editor: Alan E. Willner
  • Vol. 38, Iss. 23 — Dec. 1, 2013
  • pp: 5075–5078

Adaptive optical two-photon microscopy using autofluorescent guide stars

Xiaodong Tao, Andrew Norton, Matthew Kissel, Oscar Azucena, and Joel Kubby  »View Author Affiliations


Optics Letters, Vol. 38, Issue 23, pp. 5075-5078 (2013)
http://dx.doi.org/10.1364/OL.38.005075


View Full Text Article

Enhanced HTML    Acrobat PDF (477 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We demonstrate a fast, direct wavefront-sensing method for dynamic in vivo adaptive optical two-photon microscopy. By using a Shack–Hartmann wavefront sensor and open-loop control, the system provides high-speed wavefront measurement and correction. To measure the wavefront in the middle of a Drosophila embryo at early stages, autofluorescence from endogenous fluorophores in the yolk were used as reference guide stars. The method was tested through live imaging of a Drosophila embryo. The aberration in the middle of the embryo was measured directly for the first time. After correction, the contrast and signal intensity of the structure in the middle of the embryo was improved.

© 2013 Optical Society of America

OCIS Codes
(010.7350) Atmospheric and oceanic optics : Wave-front sensing
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(110.1080) Imaging systems : Active or adaptive optics

ToC Category:
Microscopy

History
Original Manuscript: July 29, 2013
Revised Manuscript: October 19, 2013
Manuscript Accepted: October 22, 2013
Published: November 25, 2013

Virtual Issues
Vol. 9, Iss. 2 Virtual Journal for Biomedical Optics

Citation
Xiaodong Tao, Andrew Norton, Matthew Kissel, Oscar Azucena, and Joel Kubby, "Adaptive optical two-photon microscopy using autofluorescent guide stars," Opt. Lett. 38, 5075-5078 (2013)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-38-23-5075

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

Supplementary Material


» Media 1: AVI (545 KB)     

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited