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Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 38, Iss. 23 — Dec. 1, 2013
  • pp: 5134–5137

Optical trapping of red blood cells in living animals with a water immersion objective

Min-Cheng Zhong, Lei Gong, Jin-Hua Zhou, Zi-Qiang Wang, and Yin-Mei Li  »View Author Affiliations

Optics Letters, Vol. 38, Issue 23, pp. 5134-5137 (2013)

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We demonstrate optical trapping of red blood cells (RBCs) in living animals by using a water immersion objective. First, the cells within biological tissue are mimicked by the particles immersed in aqueous solutions of glycerol. The optical forces depending on trapping depth are investigated when a parallel laser beam enters the water immersion objective. The results show that the optical forces vary with trapping depth, and the optimal trapping depth in aqueous solutions of glycerol (n=1.39) is 50 μm. Second, the optimal trapping depth in aqueous solutions of glycerol can be changed by altering the actual tube length of the water immersion objective. Finally, we achieved optical trapping and manipulation of RBCs in living mice.

© 2013 Optical Society of America

OCIS Codes
(170.3660) Medical optics and biotechnology : Light propagation in tissues
(170.4520) Medical optics and biotechnology : Optical confinement and manipulation
(220.1000) Optical design and fabrication : Aberration compensation
(350.4855) Other areas of optics : Optical tweezers or optical manipulation

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: October 9, 2013
Revised Manuscript: October 29, 2013
Manuscript Accepted: October 30, 2013
Published: November 26, 2013

Virtual Issues
Vol. 9, Iss. 2 Virtual Journal for Biomedical Optics

Min-Cheng Zhong, Lei Gong, Jin-Hua Zhou, Zi-Qiang Wang, and Yin-Mei Li, "Optical trapping of red blood cells in living animals with a water immersion objective," Opt. Lett. 38, 5134-5137 (2013)

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