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Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 38, Iss. 23 — Dec. 1, 2013
  • pp: 5184–5187

Topical dual-stain difference imaging for rapid intra-operative tumor identification in fresh specimens

Scott C. Davis, Summer L. Gibbs, Jason R. Gunn, and Brian W. Pogue  »View Author Affiliations

Optics Letters, Vol. 38, Issue 23, pp. 5184-5187 (2013)

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Assessing tumor margin status during surgery is critical to ensure complete resection of cancer tissue; however, current approaches are ineffective and often result in repeat surgery. We present an optical imaging approach for margin assessment using topical application of two fluorescent stains, one targeted to a tumor biomarker and the other a nontargeted reference, to freshly excised specimens. Computing a normalized difference image from fluorescence images of the targeted and untargeted stains suppresses the confounding effects of nonspecific uptake. Applying this approach in excised breast tumor models produced promising tumor-to-normal tissue contrasts that were significantly higher than single-targeted-stain imaging.

© 2013 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(170.4730) Medical optics and biotechnology : Optical pathology
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: August 23, 2013
Revised Manuscript: October 23, 2013
Manuscript Accepted: October 28, 2013
Published: November 27, 2013

Virtual Issues
Vol. 9, Iss. 2 Virtual Journal for Biomedical Optics

Scott C. Davis, Summer L. Gibbs, Jason R. Gunn, and Brian W. Pogue, "Topical dual-stain difference imaging for rapid intra-operative tumor identification in fresh specimens," Opt. Lett. 38, 5184-5187 (2013)

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