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Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Editor: Alan E. Willner
  • Vol. 38, Iss. 24 — Dec. 15, 2013
  • pp: 5280–5283

Comparison of line-scanned and point-scanned dual-axis confocal microscope performance

D. Wang, Y. Chen, Y. Wang, and J. T. C. Liu  »View Author Affiliations


Optics Letters, Vol. 38, Issue 24, pp. 5280-5283 (2013)
http://dx.doi.org/10.1364/OL.38.005280


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Abstract

The point-scanned dual-axis confocal (PS-DAC) microscope has been shown to exhibit superior capability to reject out-of-focus and multiply scattered light in comparison to its conventional single-axis counterpart. However, the slow frame rate (typically <5Hz) resulting from point-by-point data collection makes these systems vulnerable to motion artifacts. While video-rate point-scanned confocal microscopy is possible, a line-scanned dual-axis confocal (LS-DAC) microscope provides a simpler means of achieving high-speed imaging through line-by-line data collection, but sacrifices contrast due to loss of confocality along one dimension. Here we evaluate the performance trade-offs between an LS-DAC and PS-DAC microscope with identical spatial resolutions. Characterization experiments of the LS-DAC and PS-DAC microscopes with tissue phantoms, in reflectance mode, are shown to match results from Monte Carlo scattering simulations of the systems. Fluorescence images of mouse brain vasculature, obtained using resolution-matched LS-DAC and PS-DAC microscopes, demonstrate the comparable performance of LS-DAC and PS-DAC microscopy at shallow depths.

© 2013 Optical Society of America

OCIS Codes
(170.0180) Medical optics and biotechnology : Microscopy
(170.1790) Medical optics and biotechnology : Confocal microscopy
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.5810) Medical optics and biotechnology : Scanning microscopy
(220.4830) Optical design and fabrication : Systems design

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: September 30, 2013
Revised Manuscript: November 6, 2013
Manuscript Accepted: November 7, 2013
Published: December 4, 2013

Virtual Issues
Vol. 9, Iss. 2 Virtual Journal for Biomedical Optics

Citation
D. Wang, Y. Chen, Y. Wang, and J. T. C. Liu, "Comparison of line-scanned and point-scanned dual-axis confocal microscope performance," Opt. Lett. 38, 5280-5283 (2013)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-38-24-5280


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