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Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 38, Iss. 3 — Feb. 1, 2013
  • pp: 332–334

Combination of spectral and fluorescence imaging microscopy for wide-field in vivo analysis of microvessel blood supply and oxygenation

Jennifer A. Lee, Raymond T. Kozikowski, and Brian S. Sorg  »View Author Affiliations

Optics Letters, Vol. 38, Issue 3, pp. 332-334 (2013)

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Hyperspectral imaging of hemoglobin (Hb) saturation and first-pass fluorescence imaging of blood transit time were combined to analyze the oxygenation of and blood flow through microvessel networks. The combination imaging technique was demonstrated in a mouse dorsal window chamber model of a growing Caki-2 human renal cell carcinoma over time. Data from Hb saturation and blood supply time maps show the formation of arteriovenous malformations and shunting of blood directly from arteries to the tumor core and into veins in the periphery of the tumor. Images and data analysis show these malformations result in an oxygenated environment ideal for a tumor to proliferate.

© 2013 Optical Society of America

OCIS Codes
(170.0110) Medical optics and biotechnology : Imaging systems
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.2520) Microscopy : Fluorescence microscopy
(300.6280) Spectroscopy : Spectroscopy, fluorescence and luminescence

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: November 16, 2012
Revised Manuscript: December 19, 2012
Manuscript Accepted: December 28, 2012
Published: January 28, 2013

Virtual Issues
Vol. 8, Iss. 3 Virtual Journal for Biomedical Optics
January 28, 2013 Spotlight on Optics

Jennifer A. Lee, Raymond T. Kozikowski, and Brian S. Sorg, "Combination of spectral and fluorescence imaging microscopy for wide-field in vivo analysis of microvessel blood supply and oxygenation," Opt. Lett. 38, 332-334 (2013)

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  1. P. Carmeliet, J. Intern. Med. 255, 538 (2004). [CrossRef]
  2. S. Dufort, L. Sancey, C. Wenk, V. Josserand, and J. L. Coll, Biochim. Biophys. Acta 1798, 2266 (2010). [CrossRef]
  3. M. Wankhede, C. Dedeugd, D. W. Siemann, and B. S. Sorg, Oncol. Rep. 23, 685 (2010). [CrossRef]
  4. K. S. Oye, G. Gulati, B. A. Graff, J. V. Gaustad, K. G. Brurberg, and E. K. Rofstad, Microvasc. Res. 75, 179(2008). [CrossRef]
  5. B. S. Sorg, B. J. Moeller, O. Donovan, Y. Cao, and M. W. Dewhirst, J. Biomed. Opt. 10, 044004 (2005). [CrossRef]
  6. A. D. Bangham, M. M. Standish, and J. C. Watkens, J. Mol. Biol. 13, 238 (1965). [CrossRef]
  7. S. O. Park, M. Wankhede, Y. J. Lee, S. Choe, S. Oh, G. Walter, M. K. Raizada, B. S. Sorg, and S. P. Oh, J. Clin. Invest. 119, 3487 (2009). [CrossRef]

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