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Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 38, Iss. 8 — Apr. 15, 2013
  • pp: 1364–1366

Čerenkov excited fluorescence tomography using external beam radiation

Jennifer-Lynn Demers, Scott C. Davis, Rongxiao Zhang, David J. Gladstone, and Brian W. Pogue  »View Author Affiliations

Optics Letters, Vol. 38, Issue 8, pp. 1364-1366 (2013)

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Radiation treatment of cancer induces an optical Čerenkov emission throughout the treated volume, which could be used to excite molecular reporters in vivo, allowing molecular sensing of tissue response during fractionated therapy. In this Letter, the idea that spatial mapping of this signal can be achieved with tomographic recovery of the fluorophore distribution is tested for the first time using 6 MV photons from a linear accelerator in a heterogeneous tissue phantom. Čerenkov light excited fluorophores throughout the tissue phantom, and diffuse tomography was used to recover images. Measurements from 13 locations were used, with spectrometer detection and spectral fitting, to separate the fluorophore emission from the Čerenkov continuum. Fluorescent diffuse tomographic images showed a linear response between the concentration and the reconstructed values. The potential to apply this molecular imaging in treatment with molecular reporters appears promising.

© 2013 Optical Society of America

OCIS Codes
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(170.6960) Medical optics and biotechnology : Tomography

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: February 22, 2013
Revised Manuscript: March 21, 2013
Manuscript Accepted: March 22, 2013
Published: April 15, 2013

Virtual Issues
Vol. 8, Iss. 5 Virtual Journal for Biomedical Optics

Jennifer-Lynn Demers, Scott C. Davis, Rongxiao Zhang, David J. Gladstone, and Brian W. Pogue, "Čerenkov excited fluorescence tomography using external beam radiation," Opt. Lett. 38, 1364-1366 (2013)

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  1. S. E. Noda, T. Lautenschlaeger, M. R. Siedow, D. R. Patel, A. El-Jawahri, Y. Suzuki, J. S. Loeffler, M. R. Bussiere, and A. Chakravarti, Seminars Rad. Onc. 19, 179 (2009). [CrossRef]
  2. P. A. Čerenkov, Phys. Rev. 52, 0378 (1937). [CrossRef]
  3. J. Axelsson, S. C. Davis, D. J. Gladstone, and B. W. Pogue, Med. Phys. 38, 4127 (2011). [CrossRef]
  4. A. K. Glaser, R. Zhang, S. C. Davis, D. J. Gladstone, and B. W. Pogue, Opt. Lett. 37, 1193 (2012). [CrossRef]
  5. R. X. Zhang, A. Glaser, T. V. Esipova, S. C. Kanick, S. C. Davis, S. Vinogradov, D. Gladstone, and B. W. Pogue, Biomed. Opt. Express 3, 2381 (2012). [CrossRef]
  6. R. S. Dothager, R. J. Goiffon, E. Jackson, S. Harpstrite, and D. Piwnica-Worms, PLoS ONE 5, e13300 (2010). [CrossRef]
  7. S. C. Davis, B. W. Pogue, S. B. Tuttle, H. Dehghani, and K. D. Paulsen, J. Appl. Phys. 105, 102024 (2009). [CrossRef]
  8. H. Dehghani, M. E. Eames, P. K. Yalavarthy, S. C. Davis, S. Srinivasan, C. M. Carpenter, B. W. Pogue, and K. D. Paulsen, Commun. Numer. Methods Eng. 25, 711 (2009). [CrossRef]
  9. S. C. Davis, K. S. Samkoe, J. A. O’Hara, S. L. Gibbs-Strauss, K. D. Paulsen, and B. W. Pogue, J. Biomed. Opt. 15, 051602 (2010). [CrossRef]
  10. S. Agostinelli, J. Allison, K. Amako, J. Apostolakis, H. Araujo, P. Arce, M. Asai, D. Axen, S. Banerjee, and G. Barrand, Nucl. Instrum. Methods Phys. Res. A 506, 250 (2003). [CrossRef]
  11. P. Arce, P. Rato, M. Cañadas, and J. Lagares, in 2008 Nuclear Science Symposium, Medical Imaging Conference and 16th Room Temperature Semiconductor Detector Workshop, Dresden, Germany (IEEE, 2008), pp. 19–25.
  12. D. Razansky and V. Ntziachristos, Med. Phys. 34, 4293 (2007). [CrossRef]
  13. N. C. Deliolanis, J. Dunham, T. Wurdinger, J. L. Figueiredo, B. A. Tannous, and V. Ntziachristos, J. Biomed. Opt. 14, 030509 (2009). [CrossRef]
  14. Y. Lu, X. Zhang, A. Douraghy, D. Stout, J. Tian, T. F. Chan, and A. F. Chatziioannou, Opt. Express 17, 8062 (2009). [CrossRef]
  15. W. Cong, G. Wang, D. Kumar, Y. Liu, M. Jiang, L. V. Wang, E. A. Hoffman, G. McLennan, P. B. McCray, and J. Zabner, Opt. Express 13, 6756 (2005). [CrossRef]
  16. H. Dehghani, S. C. Davis, S. D. Jiang, B. W. Pogue, K. D. Paulsen, and M. S. Patterson, Opt. Lett. 31, 365 (2006). [CrossRef]
  17. C. Li, G. S. Mitchell, and S. R. Cherry, Opt. Lett. 35, 1109 (2010). [CrossRef]

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