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Optics Letters

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  • Editor: Xi-Cheng Zhang
  • Vol. 39, Iss. 1 — Jan. 1, 2014
  • pp: 108–110

Ultralow frequency Stokes and anti-Stokes Raman spectroscopy of single living cells and microparticles using a hot rubidium vapor filter

Jinda Lin and Yong-qing Li  »View Author Affiliations


Optics Letters, Vol. 39, Issue 1, pp. 108-110 (2014)
http://dx.doi.org/10.1364/OL.39.000108


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Abstract

We report on ultralow frequency Stokes and anti-Stokes Raman spectroscopy of single living cells and microsized particles in an aqueous medium with a frequency shift down to 10cm1 by the combination of a hot rubidium (Rb) vapor filter, a confocal pinhole, and optical trapping. A single frequency-stabilized diode laser beam at 780.2 nm is used to optically trap and excite a single living cell or microparticle, and the Rayleigh scattering light from the particle is effectively blocked with a Rb vapor cell and a confocal pinhole. Ultralow frequency Raman spectra of the trapped cells or microparticles in both Stokes and anti-Stokes regions are then measured with a single-stage CCD spectrograph.

© 2013 Optical Society of America

OCIS Codes
(170.5660) Medical optics and biotechnology : Raman spectroscopy
(300.6450) Spectroscopy : Spectroscopy, Raman
(350.4855) Other areas of optics : Optical tweezers or optical manipulation

ToC Category:
Spectroscopy

History
Original Manuscript: November 13, 2013
Manuscript Accepted: November 22, 2013
Published: December 23, 2013

Virtual Issues
Vol. 9, Iss. 3 Virtual Journal for Biomedical Optics

Citation
Jinda Lin and Yong-qing Li, "Ultralow frequency Stokes and anti-Stokes Raman spectroscopy of single living cells and microparticles using a hot rubidium vapor filter," Opt. Lett. 39, 108-110 (2014)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-39-1-108

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