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Optics Letters

Optics Letters


  • Editor: Xi-Cheng Zhang
  • Vol. 39, Iss. 10 — May. 15, 2014
  • pp: 2916–2919

Digital holographic microscopy with coupled optical fiber trap for cell measurement and manipulation

Samira Ebrahimi, Ali-Reza Moradi, Arun Anand, and Bahram Javidi  »View Author Affiliations

Optics Letters, Vol. 39, Issue 10, pp. 2916-2919 (2014)

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We present an integrated optical system for three-dimensional (3D) imaging of micrometer-sized samples, while immobilizing and manipulating the samples by means of an optical fiber trap. Optical traps allow us to apply and measure pico-Newton-sized forces, and perform detailed measurements of micrometer-sized dielectric systems in the field of biology. The integrated 3D system can be used as a major tool in the field of biophysics. The trap is built using a tapered optical fiber to enhance the effective numerical aperture of the fiber. The trapping system is mounted on a conventional microscope, in which the two eyepieces’ output ports are used as the paths of an off-axis self-referencing digital holographic microscopy (DHM) setup. The trap is calibrated using a high-speed camera, and trap stiffness is determined through the power spectrum method. The compact setup provides an elegant apparatus for temporally stable DHM for 3D imaging of optically controlled samples. Three-dimensional information and quantitative phase contrast images of the trapped samples are obtained by postprocessing the recorded digital holograms. Experiments were performed on lipids and red blood cells. Quantitative phase contrast images and temporal evolution of optical thickness of trapped samples are presented.

© 2014 Optical Society of America

OCIS Codes
(060.2370) Fiber optics and optical communications : Fiber optics sensors
(120.3890) Instrumentation, measurement, and metrology : Medical optics instrumentation
(120.5050) Instrumentation, measurement, and metrology : Phase measurement
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(280.1415) Remote sensing and sensors : Biological sensing and sensors
(350.4855) Other areas of optics : Optical tweezers or optical manipulation

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: February 10, 2014
Revised Manuscript: April 7, 2014
Manuscript Accepted: April 8, 2014
Published: May 8, 2014

Virtual Issues
Vol. 9, Iss. 7 Virtual Journal for Biomedical Optics

Samira Ebrahimi, Ali-Reza Moradi, Arun Anand, and Bahram Javidi, "Digital holographic microscopy with coupled optical fiber trap for cell measurement and manipulation," Opt. Lett. 39, 2916-2919 (2014)

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