Holographic optical tweezers (HOTs) extend optical trapping into three dimensions. Volume imaging then becomes a concern as trapped objects are easily moved out of focus of the imaging objective lens. Here we demonstrate a three-dimensional real-time interactive optical trapping, manipulating, and imaging system based on HOTs incorporated with volume holographic microscope. Intensity information about the trapped objects at multiple depths can be captured in a single measurement. This method is compatible with most imaging modes such as bright-field and fluorescence.
© 2014 Optical Society of America
Original Manuscript: March 25, 2014
Revised Manuscript: April 12, 2014
Manuscript Accepted: April 13, 2014
Published: May 15, 2014
Zhi Chen, Wensheng Chen, Hsin-yu Lu, Yves Chevallier, Nanguang Chen, George Barbastathis, and Yuan Luo, "Real-time 3D particle manipulation visualized using volume holographic gratings," Opt. Lett. 39, 3078-3081 (2014)
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