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Optics Letters

Optics Letters


  • Editor: Xi-Cheng Zhang
  • Vol. 39, Iss. 11 — Jun. 1, 2014
  • pp: 3134–3137

Temporal focusing-based multiphoton excitation microscopy via digital micromirror device

Jenq-Nan Yih, Yvonne Yuling Hu, Yong Da Sie, Li-Chung Cheng, Chi-Hsiang Lien, and Shean-Jen Chen  »View Author Affiliations

Optics Letters, Vol. 39, Issue 11, pp. 3134-3137 (2014)

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This Letter presents an enhanced temporal focusing-based multiphoton excitation (MPE) microscope in which the conventional diffraction grating is replaced by a digital micromirror device (DMD). Experimental results from imaging a thin fluorescence film show that the 4.0 μm axial resolution of the microscope is comparable with that of a setup incorporating a 600 lines / mm grating; hence, the optical sectioning ability of the proposed setup is demonstrated. Similar to a grating, the DMD diffracts illuminating light frequencies for temporal focusing; additionally, it generates arbitrary patterns. Since the DMD is placed on the image–conjugate plane of the objective lens’ focal plane, the MPE pattern can be projected on the focal plane precisely.

© 2014 Optical Society of America

OCIS Codes
(140.7090) Lasers and laser optics : Ultrafast lasers
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: January 16, 2014
Revised Manuscript: April 2, 2014
Manuscript Accepted: April 13, 2014
Published: May 19, 2014

Virtual Issues
Vol. 9, Iss. 8 Virtual Journal for Biomedical Optics

Jenq-Nan Yih, Yvonne Yuling Hu, Yong Da Sie, Li-Chung Cheng, Chi-Hsiang Lien, and Shean-Jen Chen, "Temporal focusing-based multiphoton excitation microscopy via digital micromirror device," Opt. Lett. 39, 3134-3137 (2014)

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