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Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Editor: Xi-Cheng Zhang
  • Vol. 39, Iss. 12 — Jun. 15, 2014
  • pp: 3523–3526

Immune cell imaging using multi-spectral optoacoustic tomography

Stratis Tzoumas, Angelika Zaremba, Uwe Klemm, Antonio Nunes, Karin Schaefer, and Vasilis Ntziachristos  »View Author Affiliations


Optics Letters, Vol. 39, Issue 12, pp. 3523-3526 (2014)
http://dx.doi.org/10.1364/OL.39.003523


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Abstract

Multispectral optoacoustic tomography (MSOT) offers the potential to image in high-resolution cells tagged with optical labels. In contrast to single wavelength imaging, multispectral excitation and spectral unmixing can differentiate labeled moieties over tissue absorption in the absence of background measurements. This feature can enable longitudinal cellular biology studies well beyond the depths reached by optical microscopy. However, the relation between spectrally resolved fluorescently labeled cells and optoacoustic detection has not been systematically investigated. Herein, we measured titrations of fluorescently labeled cells and establish the optoacoustic signal generated by these cells as a function of cell number and across different cell types. We then assess the MSOT sensitivity to resolve cells implanted in animals.

© 2014 Optical Society of America

OCIS Codes
(170.1530) Medical optics and biotechnology : Cell analysis
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.5120) Medical optics and biotechnology : Photoacoustic imaging

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: March 24, 2014
Manuscript Accepted: April 26, 2014
Published: June 9, 2014

Virtual Issues
Vol. 9, Iss. 8 Virtual Journal for Biomedical Optics

Citation
Stratis Tzoumas, Angelika Zaremba, Uwe Klemm, Antonio Nunes, Karin Schaefer, and Vasilis Ntziachristos, "Immune cell imaging using multi-spectral optoacoustic tomography," Opt. Lett. 39, 3523-3526 (2014)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-39-12-3523


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