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Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Editor: Xi-Cheng Zhang
  • Vol. 39, Iss. 12 — Jun. 15, 2014
  • pp: 3682–3685

Confocal line scanning of a Bessel beam for fast 3D Imaging

P. Zhang, M. E. Phipps, P. M. Goodwin, and J. H. Werner  »View Author Affiliations


Optics Letters, Vol. 39, Issue 12, pp. 3682-3685 (2014)
http://dx.doi.org/10.1364/OL.39.003682


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Abstract

We have developed a light-sheet illumination microscope that can perform fast 3D imaging of transparent biological samples with inexpensive visible lasers and a single galvo mirror (GM). The light-sheet is created by raster scanning a Bessel beam with a GM, with this same GM also being used to rescan the fluorescence across a chip of a camera to construct an image in real time. A slit is used to reject out-of-focus fluorescence such that the image formed in real time has minimal contribution from the sidelobes of the Bessel beam. Compared with two-photon Bessel beam excitation or other confocal line-scanning approaches, our method is of lower cost, is simpler, and does not require calibration and synchronization of multiple GMs. We demonstrated the optical sectioning and out-of-focus background rejection capabilities of this microscope by imaging fluorescently labeled actin filaments in fixed 3T3 cells.

© 2014 Optical Society of America

OCIS Codes
(170.1790) Medical optics and biotechnology : Confocal microscopy
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:
Microscopy

History
Original Manuscript: March 13, 2014
Manuscript Accepted: May 9, 2014
Published: June 13, 2014

Virtual Issues
Vol. 9, Iss. 8 Virtual Journal for Biomedical Optics

Citation
P. Zhang, M. E. Phipps, P. M. Goodwin, and J. H. Werner, "Confocal line scanning of a Bessel beam for fast 3D Imaging," Opt. Lett. 39, 3682-3685 (2014)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-39-12-3682


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References

  1. J. Pawley, Handbook of Biological Confocal Microscopy (Springer, 2006).
  2. W. Denk, J. Strickler, and W. Webb, Science 248, 73 (1990). [CrossRef]
  3. P. T. C. So, C. Y. Dong, B. R. Masters, and K. M. Berland, Ann. Rev. Biomed. Eng. 2, 399 (2000).
  4. J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer, Science 305, 1007 (2004). [CrossRef]
  5. J. Mertz and J. Kim, J. Biomed. Opt. 15, 016027 (2010). [CrossRef]
  6. T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, Nat. Methods 8, 417 (2011). [CrossRef]
  7. J. G. Ritter, R. Veith, A. Veenendaal, J. P. Siebrasse, and U. Kubitscheck, PLoS ONE 5, e11639 (2010). [CrossRef]
  8. J. C. M. Gebhardt, D. M. Suter, R. Roy, Z. W. Zhao, A. R. Chapman, S. Basu, T. Maniatis, and X. S. Xie, Nat. Methods 10, 421 (2013). [CrossRef]
  9. F. Cella Zanacchi, Z. Lavagnino, M. Perrone Donnorso, A. Del Bue, L. Furia, M. Faretta, and A. Diaspro, Nat. Methods 8, 1047 (2011). [CrossRef]
  10. F. O. Fahrbach and A. Rohrbach, Nat. Commun. 3, 632 (2012). [CrossRef]
  11. F. O. Fahrbach, V. Gurchenkov, K. Alessandri, P. Nassoy, and A. Rohrbach, Opt. Express 21, 13824 (2013). [CrossRef]
  12. L. Silvestri, A. Bria, L. Sacconi, G. Iannello, and F. S. Pavone, Opt. Express 20, 20582 (2012). [CrossRef]
  13. E. Baumgart and U. Kubitscheck, Opt. Express 20, 21805 (2012). [CrossRef]
  14. R. M. Herman and T. A. Wiggins, J. Opt. Soc. Am. A 8, 932 (1991). [CrossRef]
  15. P. Zhang, P. M. Goodwin, and J. H. Werner, Proc. SPIE 8947, 89471K (2014). [CrossRef]
  16. M. Born and E. Wolf, Principles of Optics (Cambridge University, 1999).
  17. P. Zhang, P. M. Goodwin, and J. H. Werner, Opt. Express 22, 12398 (2014). [CrossRef]
  18. J. J. Han, A. P. Shreve, and J. H. Werner, in Characterization of Materials (Wiley, 2012), pp. 1026–1039.
  19. E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, Science 313, 1642 (2006). [CrossRef]
  20. M. J. Rust, M. Bates, and X. Zhuang, Nat. Methods 3, 793 (2006). [CrossRef]
  21. C. S. Smith, N. Joseph, B. Rieger, and K. A. Lidke, Nat. Methods 7, 373 (2010). [CrossRef]
  22. S. Quirin, S. R. P. Pavani, and R. Piestun, Proc. Natl. Acad. Sci. 109, 675 (2012). [CrossRef]
  23. Y. Lill and B. Hecht, Appl. Phys. Lett. 84, 1665 (2004). [CrossRef]

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