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Optics Letters

Optics Letters


  • Editor: Xi-Cheng Zhang
  • Vol. 39, Iss. 12 — Jun. 15, 2014
  • pp: 3682–3685

Confocal line scanning of a Bessel beam for fast 3D Imaging

P. Zhang, M. E. Phipps, P. M. Goodwin, and J. H. Werner  »View Author Affiliations

Optics Letters, Vol. 39, Issue 12, pp. 3682-3685 (2014)

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We have developed a light-sheet illumination microscope that can perform fast 3D imaging of transparent biological samples with inexpensive visible lasers and a single galvo mirror (GM). The light-sheet is created by raster scanning a Bessel beam with a GM, with this same GM also being used to rescan the fluorescence across a chip of a camera to construct an image in real time. A slit is used to reject out-of-focus fluorescence such that the image formed in real time has minimal contribution from the sidelobes of the Bessel beam. Compared with two-photon Bessel beam excitation or other confocal line-scanning approaches, our method is of lower cost, is simpler, and does not require calibration and synchronization of multiple GMs. We demonstrated the optical sectioning and out-of-focus background rejection capabilities of this microscope by imaging fluorescently labeled actin filaments in fixed 3T3 cells.

© 2014 Optical Society of America

OCIS Codes
(170.1790) Medical optics and biotechnology : Confocal microscopy
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:

Original Manuscript: March 13, 2014
Manuscript Accepted: May 9, 2014
Published: June 13, 2014

Virtual Issues
Vol. 9, Iss. 8 Virtual Journal for Biomedical Optics

P. Zhang, M. E. Phipps, P. M. Goodwin, and J. H. Werner, "Confocal line scanning of a Bessel beam for fast 3D Imaging," Opt. Lett. 39, 3682-3685 (2014)

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