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Optics Letters

Optics Letters


  • Editor: Xi-Cheng Zhang
  • Vol. 39, Iss. 13 — Jul. 1, 2014
  • pp: 3907–3910

Entropy analysis reveals a simple linear relation between laser speckle and blood flow

Peng Miao, Zhen Chao, Yiguang Zhang, Nan Li, and Nitish V. Thakor  »View Author Affiliations

Optics Letters, Vol. 39, Issue 13, pp. 3907-3910 (2014)

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Dynamic laser speckles contain motion information of scattering particles which can be estimated by laser speckle contrast analysis (LASCA). In this work, an entropy-based method was proposed to provide a more robust estimation of motion speed. An in vitro flow simulation experiment confirmed a simple linear relation between entropy, exposure time, and speed. A multimodality optical imaging setup is developed to validate the advantages of the entropy method based on laser speckle imaging, green light imaging, and fluorescence imaging. The entropy method overcomes traditional LASCA with less noisy interference, and extracts more visible and detailed vasculatures in vivo. Furthermore, the entropy method provides a more accurate estimation and a stable pattern of blood flow activations in the rat’s somatosensory area under multitrial hand paw stimulations.

© 2014 Optical Society of America

OCIS Codes
(120.6150) Instrumentation, measurement, and metrology : Speckle imaging
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.6480) Medical optics and biotechnology : Spectroscopy, speckle
(170.2655) Medical optics and biotechnology : Functional monitoring and imaging

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: April 23, 2014
Revised Manuscript: May 19, 2014
Manuscript Accepted: May 28, 2014
Published: June 25, 2014

Virtual Issues
Vol. 9, Iss. 9 Virtual Journal for Biomedical Optics

Peng Miao, Zhen Chao, Yiguang Zhang, Nan Li, and Nitish V. Thakor, "Entropy analysis reveals a simple linear relation between laser speckle and blood flow," Opt. Lett. 39, 3907-3910 (2014)

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