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Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Editor: Xi-Cheng Zhang
  • Vol. 39, Iss. 15 — Aug. 1, 2014
  • pp: 4290–4293

What structural length scales can be detected by the spectral variance of a microscope image?

Lusik Cherkezyan, Hariharan Subramanian, and Vadim Backman  »View Author Affiliations


Optics Letters, Vol. 39, Issue 15, pp. 4290-4293 (2014)
http://dx.doi.org/10.1364/OL.39.004290


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Abstract

A spectroscopic microscope, configured to detect interference spectra of backscattered light in the far zone, quantifies the statistics of refractive-index (RI) distribution via the spectral variance (Σ˜2) of the acquired bright-field image. Its sensitivity to subtle structural changes within weakly scattering, label-free media at subdiffraction scales shows great promise in fields from material science to medical diagnostics. We further investigate the length-scale sensitivity of Σ˜ and reveal that, in theory, it can detect RI fluctuations at any spatial frequency whatsoever. Based on a 5% noise floor, Σ˜ detects scales from 22 to 200–700 nm (exact values depend on sample structure and thickness). In an example involving mass-density distribution characteristic of biological cell nuclei, we suggest the level of chromatin organization, which can be quantified via Σ˜.

© 2014 Optical Society of America

OCIS Codes
(170.1530) Medical optics and biotechnology : Cell analysis
(180.0180) Microscopy : Microscopy
(290.0290) Scattering : Scattering
(300.0300) Spectroscopy : Spectroscopy

ToC Category:
Microscopy

History
Original Manuscript: March 10, 2014
Revised Manuscript: June 5, 2014
Manuscript Accepted: June 12, 2014
Published: July 16, 2014

Citation
Lusik Cherkezyan, Hariharan Subramanian, and Vadim Backman, "What structural length scales can be detected by the spectral variance of a microscope image?," Opt. Lett. 39, 4290-4293 (2014)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-39-15-4290

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