OSA's Digital Library

Optics Letters

Optics Letters


  • Editor: Xi-Cheng Zhang
  • Vol. 39, Iss. 15 — Aug. 1, 2014
  • pp: 4424–4426

Measuring blood velocity using correlative spectrally encoded flow cytometry

Tal Elhanan and Dvir Yelin  »View Author Affiliations

Optics Letters, Vol. 39, Issue 15, pp. 4424-4426 (2014)

View Full Text Article

Enhanced HTML    Acrobat PDF (333 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



Spectrally encoded flow cytometry (SEFC) is a promising technique for imaging blood in the microcirculation. Yet, the dependency of one of the axes of the image on time prevents effective quantification of essential clinical parameters. Here, we address this challenge by splitting the optical path in an SEFC system into two parallel imaging lines, followed by straightforward data analysis for recovering the flow speed from the multiplexed data. The method is demonstrated by measuring the flow velocity of latex beads and blood cells in vitro. The system allows real-time velocity measurements of up to 11.7mm/s at high spatial resolution, and could be integrated into existing SEFC systems for effectively measuring blood parameters in small capillary vessels.

© 2014 Optical Society of America

OCIS Codes
(120.3890) Instrumentation, measurement, and metrology : Medical optics instrumentation
(170.1790) Medical optics and biotechnology : Confocal microscopy
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(280.2490) Remote sensing and sensors : Flow diagnostics

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: May 6, 2014
Revised Manuscript: June 24, 2014
Manuscript Accepted: June 25, 2014
Published: July 23, 2014

Virtual Issues
Vol. 9, Iss. 10 Virtual Journal for Biomedical Optics

Tal Elhanan and Dvir Yelin, "Measuring blood velocity using correlative spectrally encoded flow cytometry," Opt. Lett. 39, 4424-4426 (2014)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. V. Hoffbrand and P. Moss, Essential Haematology (Wiley, 2011), Vol. 39.
  2. C. D. Jennings and K. A. Foon, Blood 90, 2863 (1997).
  3. B. J. Bain, N. Engl. J. Med. 353, 498 (2005). [CrossRef]
  4. C. Briggs, S. Kimber, and L. Green, Br. J. Haematol. 158, 679 (2012). [CrossRef]
  5. B. A. Ekberg, U. D. Larsen, and N. Fogh-Andersen, Point Care 4, 64 (2005).
  6. C. van Berkel, J. D. Gwyer, S. Deane, N. Green, J. Holloway, V. Hollis, and H. Morgan, Lab Chip 11, 1249 (2011). [CrossRef]
  7. I. Yoshiya, Y. Shimada, and K. Tanaka, Med. Biol. Eng. Comput. 18, 27 (1980). [CrossRef]
  8. V. P. Zharov, E. I. Galanzha, and V. V. Tuchin, Opt. Lett. 30, 628 (2005). [CrossRef]
  9. W. Groner, J. W. Winkelman, A. G. Harris, C. Ince, G. J. Bouma, K. Messmer, and R. G. Nadeau, Nat. Med. 5, 1209 (1999). [CrossRef]
  10. L. Golan and D. Yelin, Opt. Lett. 35, 2218 (2010). [CrossRef]
  11. J. D. Briers and S. Webster, J. Biomed. Opt. 1, 174 (1996). [CrossRef]
  12. A. K. Murray, A. L. Herrick, and T. A. King, Rheumatology 43, 1210 (2004). [CrossRef]
  13. H. Wayland and P. C. Johnson, J. Appl. Physiol. 22, 333 (1967).
  14. L. Golan, D. Yeheskely-Hayon, L. Minai, E. J. Dann, and D. Yelin, Biomed. Opt. Express 3, 1455 (2012). [CrossRef]
  15. C. J. Sheppard, W. Gong, and K. Si, Opt. Express 16, 17031 (2008). [CrossRef]
  16. R. Fox, A. McDonald, and P. J. Pritchard, Introduction to Fluid Mechanics, 6th ed. (Wiley, 2006).
  17. A. S. Popel and P. C. Johnson, Annu. Rev. Fluid Mech. 37, 43 (2005). [CrossRef]
  18. C. E. Willert and M. Gharib, Exp. Fluids 10, 181 (1991). [CrossRef]
  19. D. P. Hart, J. Vis. 3, 187 (2000).
  20. J. Westerweel, in Laser Techniques Applied to Fluid Mechanics, R. J. Adrian, D. F. G. Durao, F. Durst, M. V. Heitor, M. Maeda, and J. H. Whitelaw, eds. (Springer, 2000), pp. 37–55.

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


Fig. 1. Fig. 2. Fig. 3.
Fig. 4.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited