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Optics Letters

Optics Letters


  • Editor: Xi-Cheng Zhang
  • Vol. 39, Iss. 15 — Aug. 1, 2014
  • pp: 4529–4532

Demonstration of subtraction imaging in confocal microscopy with vector beams

Susumu Segawa, Yuichi Kozawa, and Shunichi Sato  »View Author Affiliations

Optics Letters, Vol. 39, Issue 15, pp. 4529-4532 (2014)

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The subtraction imaging in confocal microscopy is demonstrated by using two vector beams with radial and azimuthal polarizations. The longitudinal electric component, which appears near the focus of a radially polarized beam under the tight focusing condition and produces a smaller focal spot, is effectively extracted by the subtraction using an azimuthally polarized beam. This subtraction imaging with vector beams provides the improvement of the lateral resolution in confocal microscopy without the degradation due to the excess subtraction.

© 2014 Optical Society of America

OCIS Codes
(170.3010) Medical optics and biotechnology : Image reconstruction techniques
(180.1790) Microscopy : Confocal microscopy
(260.5430) Physical optics : Polarization

ToC Category:

Original Manuscript: April 16, 2014
Revised Manuscript: June 19, 2014
Manuscript Accepted: June 27, 2014
Published: July 29, 2014

Virtual Issues
Vol. 9, Iss. 10 Virtual Journal for Biomedical Optics

Susumu Segawa, Yuichi Kozawa, and Shunichi Sato, "Demonstration of subtraction imaging in confocal microscopy with vector beams," Opt. Lett. 39, 4529-4532 (2014)

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