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Optics Letters

Optics Letters


  • Editor: Xi-Cheng Zhang
  • Vol. 39, Iss. 17 — Sep. 1, 2014
  • pp: 5192–5195

Near-infrared optical-resolution photoacoustic microscopy

Pengfei Hai, Junjie Yao, Konstantin I. Maslov, Yong Zhou, and Lihong V. Wang  »View Author Affiliations

Optics Letters, Vol. 39, Issue 17, pp. 5192-5195 (2014)

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Compared with visible light (380–700 nm), near-infrared light (700–1400 nm) undergoes weaker optical attenuation in biological tissue; thus, it can penetrate deeper. Herein, we demonstrate near-infrared optical-resolution photoacoustic microscopy (NIR-OR-PAM) with 1046 nm illumination. A penetration depth of 3.2 mm was achieved in chicken breast tissue ex vivo using optical fluence within the American National Standards Institute (ANSI) limit (100mJ/cm2). Beyond 0.6mm deep in chicken breast tissue, NIR-OR-PAM has shown finer resolution than the visible counterpart with 570 nm illumination. The deep imaging capability of NIR-OR-PAM was validated in both a mouse ear and a mouse brain. NIR-OR-PAM of possible lipid contrast was explored as well.

© 2014 Optical Society of America

OCIS Codes
(110.3080) Imaging systems : Infrared imaging
(170.5120) Medical optics and biotechnology : Photoacoustic imaging
(180.0180) Microscopy : Microscopy

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: May 9, 2014
Revised Manuscript: July 25, 2014
Manuscript Accepted: August 1, 2014
Published: August 28, 2014

Virtual Issues
Vol. 9, Iss. 11 Virtual Journal for Biomedical Optics

Pengfei Hai, Junjie Yao, Konstantin I. Maslov, Yong Zhou, and Lihong V. Wang, "Near-infrared optical-resolution photoacoustic microscopy," Opt. Lett. 39, 5192-5195 (2014)

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  1. L. V. Wang and S. Hu, Science 335, 1458 (2012). [CrossRef]
  2. J. Yao and L. V. Wang, Laser Photonics Rev. 7, 758 (2013). [CrossRef]
  3. J. Yao, L. Wang, C. Li, C. Zhang, and L. V. Wang, Phys. Rev. Lett. 112, 014302 (2014). [CrossRef]
  4. J. Liang, Y. Zhou, K. I. Maslov, and L. V. Wang, J. Biomed. Opt. 18, 096004 (2013). [CrossRef]
  5. R. J. Paproski, A. E. Forbrich, K. Wachowicz, M. M. Hitt, and R. J. Zemp, Biomed. Opt. Express 2, 771 (2011). [CrossRef]
  6. K. Maslov, H. F. Zhang, S. Hu, and L. V. Wang, Opt. Lett. 33, 929 (2008). [CrossRef]
  7. Y. Liu, C. Zhang, and L. V. Wang, J. Biomed. Opt. 17, 126014 (2012). [CrossRef]
  8. Z. Xie, S. Chen, T. Ling, L. J. Guo, P. L. Carson, and X. Wang, Opt. Express 19, 9027 (2011). [CrossRef]
  9. C. Zhang, Y. Zhang, D. K. Yao, Y. Xia, and L. V. Wang, J. Biomed. Opt. 18, 020504 (2013). [CrossRef]
  10. K. Homan, S. Kim, Y.-S. Chen, B. Wang, S. Mallidi, and S. Emelianov, Opt. Lett. 35, 2663 (2010). [CrossRef]
  11. Z. Xu, C. H. Li, and L. V. Wang, J. Biomed. Opt. 15, 036019 (2010). [CrossRef]
  12. T. J. Allen, A. Hall, A. P. Dhillon, J. S. Owen, and P. C. Beard, J. Biomed. Opt. 17, 061209 (2012). [CrossRef]
  13. R. L. P. van Veen, H. J. C. M. Sterenborg, A. Pifferi, A. Torricelli, and R. Cubeddu, in Biomedical Topical Meeting, OSA Technical Digest (2004), paper SF4.
  14. G. M. Hale and M. R. Querry, Appl. Opt. 12, 555 (1973). [CrossRef]
  15. “American national standard for the safe use of lasers,” (2007).
  16. L. Wang, K. Maslov, J. Yao, B. Rao, and L. V. Wang, Opt. Lett. 36, 139 (2011). [CrossRef]
  17. C. W. Freudiger, W. Min, B. G. Saar, S. Lu, G. R. Holtom, C. He, J. C. Tsai, J. X. Kang, and X. S. Xie, Science 322, 1857 (2008). [CrossRef]
  18. T. Matthews, C. Zhang, D. Yao, K. Maslov, and L. V. Wang, J. Biomed. Opt. 19, 016004 (2014). [CrossRef]
  19. S. Sato, M. Ogura, M. Ishihara, S. Kawauchi, T. Arai, T. Matsui, A. Kurita, M. Obara, M. Kikuchi, and H. Ashida, Lasers Surg. Med. 29, 464 (2001). [CrossRef]
  20. J. Yao, C. H. Huang, L. Wang, J. M. Yang, L. Gao, K. I. Maslov, J. Zou, and L. V. Wang, J. Biomed. Opt. 17, 0805051 (2012).
  21. H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, Nat. Biotechnol. 24, 848 (2006). [CrossRef]
  22. J. Yao, K. I. Maslov, Y. Zhang, Y. Xia, and L. V. Wang, J. Biomed. Opt. 16, 076003 (2011). [CrossRef]

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