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Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Editor: Xi-Cheng Zhang
  • Vol. 39, Iss. 4 — Feb. 15, 2014
  • pp: 1053–1056

OPTiSPIM: integrating optical projection tomography in light sheet microscopy extends specimen characterization to nonfluorescent contrasts

Jürgen Mayer, Alexandre Robert-Moreno, Renzo Danuser, Jens V. Stein, James Sharpe, and Jim Swoger  »View Author Affiliations


Optics Letters, Vol. 39, Issue 4, pp. 1053-1056 (2014)
http://dx.doi.org/10.1364/OL.39.001053


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Abstract

Mesoscopic 3D imaging has become a widely used optical imaging technique to visualize intact biological specimens. Selective plane illumination microscopy (SPIM) visualizes samples up to a centimeter in size with micrometer resolution by 3D data stitching but is limited to fluorescent contrast. Optical projection tomography (OPT) works with fluorescent and nonfluorescent contrasts, but its resolution is limited in large samples. We present a hybrid setup (OPTiSPIM) combining the advantages of each technique. The combination of fluorescent and nonfluorescent high-resolution 3D data into integrated datasets enables a more extensive representation of mesoscopic biological samples. The modular concept of the OPTiSPIM facilitates incorporation of the transmission OPT modality into already established light sheet based imaging setups.

© 2014 Optical Society of America

OCIS Codes
(110.6880) Imaging systems : Three-dimensional image acquisition
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.3010) Medical optics and biotechnology : Image reconstruction techniques
(110.6955) Imaging systems : Tomographic imaging

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: November 26, 2013
Manuscript Accepted: January 3, 2014
Published: February 13, 2014

Virtual Issues
Vol. 9, Iss. 4 Virtual Journal for Biomedical Optics

Citation
Jürgen Mayer, Alexandre Robert-Moreno, Renzo Danuser, Jens V. Stein, James Sharpe, and Jim Swoger, "OPTiSPIM: integrating optical projection tomography in light sheet microscopy extends specimen characterization to nonfluorescent contrasts," Opt. Lett. 39, 1053-1056 (2014)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-39-4-1053


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