Visible light Zernike phase contrast (ZPC) microscopy is a well established method for imaging weakly absorbing samples. The method is also used with hard x-ray photon energies for structural evaluation of material science and biological applications. However, the method suffers from artifacts that are inherent for the Zernike image formation. In this Letter, we investigate their origin and experimentally show how to suppress them in x-ray full-field ZPC microscopy based on diffractive x-ray optics.
© 2014 Optical Society of America
Original Manuscript: November 21, 2013
Revised Manuscript: February 7, 2014
Manuscript Accepted: February 10, 2014
Published: March 12, 2014
Vol. 9, Iss. 5 Virtual Journal for Biomedical Optics
Ismo Vartiainen, Rajmund Mokso, Marco Stampanoni, and Christian David, "Halo suppression in full-field x-ray Zernike phase contrast microscopy," Opt. Lett. 39, 1601-1604 (2014)