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Optics Letters

Optics Letters


  • Editor: Xi-Cheng Zhang
  • Vol. 39, Iss. 7 — Apr. 1, 2014
  • pp: 1819–1822

Hybrid multiphoton and optoacoustic microscope

George J. Tserevelakis, Dominik Soliman, Murad Omar, and Vasilis Ntziachristos  »View Author Affiliations

Optics Letters, Vol. 39, Issue 7, pp. 1819-1822 (2014)

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We present a hybrid microscope combining multiphoton microscopy incorporating second-harmonic generation contrast and optical-resolution optoacoustic (photoacoustic) microscopy. We study the relative performance of the two systems and investigate the complementarity of contrast by demonstrating the label-free imaging capabilities of the hybrid microscope on zebrafish larvae ex vivo, concurrently visualizing the fish musculature and melanocytes. This implementation can prove useful in multiparametric microscopy studies, enabling broader information to be collected from biological specimens.

© 2014 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(110.5120) Imaging systems : Photoacoustic imaging
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Imaging Systems

Original Manuscript: January 10, 2014
Manuscript Accepted: February 8, 2014
Published: March 20, 2014

Virtual Issues
Vol. 9, Iss. 6 Virtual Journal for Biomedical Optics

George J. Tserevelakis, Dominik Soliman, Murad Omar, and Vasilis Ntziachristos, "Hybrid multiphoton and optoacoustic microscope," Opt. Lett. 39, 1819-1822 (2014)

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