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Optics Letters

Optics Letters


  • Editor: Xi-Cheng Zhang
  • Vol. 39, Iss. 7 — Apr. 1, 2014
  • pp: 1897–1900

Experimental and simulation study of the wavelength dependent second harmonic generation of collagen in scattering tissues

Gunnsteinn Hall, Karissa B. Tilbury, Kirby R. Campbell, Kevin W. Eliceiri, and Paul J. Campagnola  »View Author Affiliations

Optics Letters, Vol. 39, Issue 7, pp. 1897-1900 (2014)

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We report on the wavelength dependence of second harmonic generation (SHG) of collagen in scattering tissues over the wavelength range of 800–1200 nm. The study incorporates inclusion of the molecular hyperpolarizability β of collagen and optical scattering, both of which are wavelength dependent. Using 3D SHG imaging and Monte Carlo simulations, we find the wavelength dependence of β is not well described by a two-state model based on known absorption bands. We further find that longer wavelength excitation is inefficient as the reduction in scattering is overcome by the decreased β far from resonance and the optimal excitation is within the 800–900 nm range. The impact is larger for backward collected SHG.

© 2014 Optical Society of America

OCIS Codes
(180.6900) Microscopy : Three-dimensional microscopy
(190.2620) Nonlinear optics : Harmonic generation and mixing
(180.4315) Microscopy : Nonlinear microscopy
(170.6935) Medical optics and biotechnology : Tissue characterization

ToC Category:

Original Manuscript: November 26, 2013
Revised Manuscript: February 10, 2014
Manuscript Accepted: February 17, 2014
Published: March 21, 2014

Virtual Issues
Vol. 9, Iss. 6 Virtual Journal for Biomedical Optics

Gunnsteinn Hall, Karissa B. Tilbury, Kirby R. Campbell, Kevin W. Eliceiri, and Paul J. Campagnola, "Experimental and simulation study of the wavelength dependent second harmonic generation of collagen in scattering tissues," Opt. Lett. 39, 1897-1900 (2014)

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