A novel and efficient architecture of a structured-illumination digital holographic microscope (DHM) is presented. As the DHM operates at the diffraction limit, its spatial resolution on label-free imaging of transparent samples is improved by illuminating the sample with a structured illumination produced by a Fresnel’s biprism. The theoretical analysis of the method forecasts a twofold improvement of the spatial resolution. The proposed method requires only two images to improve the spatial resolution, which eases the process of unmixing the high-resolution components by means of an unknown phase-shift procedure. Numerical modeling and experimental results validate the theoretical findings.
© 2014 Optical Society of America
Original Manuscript: October 3, 2013
Revised Manuscript: February 24, 2014
Manuscript Accepted: March 7, 2014
Published: March 27, 2014
Vol. 9, Iss. 6 Virtual Journal for Biomedical Optics
Emilio Sánchez-Ortiga, Manuel Martínez-Corral, Genaro Saavedra, and Jorge Garcia-Sucerquia, "Enhancing spatial resolution in digital holographic microscopy by biprism structured illumination," Opt. Lett. 39, 2086-2089 (2014)