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Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Editor: Xi-Cheng Zhang
  • Vol. 39, Iss. 7 — Apr. 1, 2014
  • pp: 2117–2120

Fully motorized optical-resolution photoacoustic microscopy

Lei Li, Chenghung Yeh, Song Hu, Lidai Wang, Brian T. Soetikno, Ruimin Chen, Qifa Zhou, K. Kirk Shung, Konstantin I. Maslov, and Lihong V. Wang  »View Author Affiliations


Optics Letters, Vol. 39, Issue 7, pp. 2117-2120 (2014)
http://dx.doi.org/10.1364/OL.39.002117


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Abstract

We have developed fully motorized optical-resolution photoacoustic microscopy (OR-PAM), which integrates five complementary scanning modes and simultaneously provides a high imaging speed and a wide field of view (FOV) with 2.6 μm lateral resolution. With one-dimensional (1D) motion-mode mechanical scanning, we measured the blood flow through a cross section of a blood vessel in vivo. With two-dimensional (2D) optical scanning at a laser repetition rate of 40 kHz, we achieved a 2 kHz B-scan rate over a range of 50 μm with 20 A-lines and 50 Hz volumetric-scan rate over a FOV of 50μm×50μm with 400 A-lines, which enabled real-time tracking of cellular dynamics in vivo. With synchronized 1D optical and 2D mechanical hybrid scanning, we imaged a 10mm×8mm FOV within three minutes, which is 20 times faster than the conventional mechanical scan in our second-generation OR-PAM. With three-dimensional mechanical contour scanning, we maintained the optimal signal-to-noise ratio and spatial resolution of OR-PAM while imaging objects with uneven surfaces, which is essential for quantitative studies.

© 2014 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.5120) Medical optics and biotechnology : Photoacoustic imaging
(180.5810) Microscopy : Scanning microscopy
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:
Microscopy

History
Original Manuscript: December 11, 2013
Manuscript Accepted: February 8, 2014
Published: March 28, 2014

Virtual Issues
Vol. 9, Iss. 6 Virtual Journal for Biomedical Optics

Citation
Lei Li, Chenghung Yeh, Song Hu, Lidai Wang, Brian T. Soetikno, Ruimin Chen, Qifa Zhou, K. Kirk Shung, Konstantin I. Maslov, and Lihong V. Wang, "Fully motorized optical-resolution photoacoustic microscopy," Opt. Lett. 39, 2117-2120 (2014)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-39-7-2117


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