We demonstrate a common-path tomographic diffractive microscopy technique for three-dimensional (3D) refractive-index (RI) imaging of unstained living cells. A diffraction grating is utilized to generate a reference beam that traverses a blank region of the sample in a common-path off-axis interferometry setup. Single-shot phase images captured at multiple illumination angles are used for 3D RI reconstruction based on optical diffraction tomography. The common-path configuration shows lower temporal phase fluctuations and better RI resolution than a Mach–Zehnder configuration. 3D subcellular RI distributions of live HeLa cells are quantified.
© 2014 Optical Society of America
Original Manuscript: January 2, 2014
Revised Manuscript: March 9, 2014
Manuscript Accepted: March 10, 2014
Published: March 31, 2014
Vol. 9, Iss. 6 Virtual Journal for Biomedical Optics
Wei-Chen Hsu, Jing-Wei Su, Te-Yu Tseng, and Kung-Bin Sung, "Tomographic diffractive microscopy of living cells based on a common-path configuration," Opt. Lett. 39, 2210-2213 (2014)