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Optics Letters

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  • Editor: Xi-Cheng Zhang
  • Vol. 39, Iss. 7 — Apr. 1, 2014
  • pp: 2210–2213

Tomographic diffractive microscopy of living cells based on a common-path configuration

Wei-Chen Hsu, Jing-Wei Su, Te-Yu Tseng, and Kung-Bin Sung  »View Author Affiliations


Optics Letters, Vol. 39, Issue 7, pp. 2210-2213 (2014)
http://dx.doi.org/10.1364/OL.39.002210


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Abstract

We demonstrate a common-path tomographic diffractive microscopy technique for three-dimensional (3D) refractive-index (RI) imaging of unstained living cells. A diffraction grating is utilized to generate a reference beam that traverses a blank region of the sample in a common-path off-axis interferometry setup. Single-shot phase images captured at multiple illumination angles are used for 3D RI reconstruction based on optical diffraction tomography. The common-path configuration shows lower temporal phase fluctuations and better RI resolution than a Mach–Zehnder configuration. 3D subcellular RI distributions of live HeLa cells are quantified.

© 2014 Optical Society of America

OCIS Codes
(120.3180) Instrumentation, measurement, and metrology : Interferometry
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.0180) Microscopy : Microscopy

ToC Category:
Imaging Systems

History
Original Manuscript: January 2, 2014
Revised Manuscript: March 9, 2014
Manuscript Accepted: March 10, 2014
Published: March 31, 2014

Virtual Issues
Vol. 9, Iss. 6 Virtual Journal for Biomedical Optics

Citation
Wei-Chen Hsu, Jing-Wei Su, Te-Yu Tseng, and Kung-Bin Sung, "Tomographic diffractive microscopy of living cells based on a common-path configuration," Opt. Lett. 39, 2210-2213 (2014)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-39-7-2210


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References

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