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Optics Letters

Optics Letters


  • Editor: Xi-Cheng Zhang
  • Vol. 39, Iss. 7 — Apr. 1, 2014
  • pp: 2214–2217

Optimization of in vivo spectroscopic photoacoustic imaging by smart optical wavelength selection

Geoffrey P. Luke and Stanislav Y. Emelianov  »View Author Affiliations

Optics Letters, Vol. 39, Issue 7, pp. 2214-2217 (2014)

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Spectroscopic photoacoustic (sPA) imaging is an emerging biomedical imaging modality which can be used to simultaneously identify multiple optical absorbers in tissue. With current technology, the image acquisition time is limited primarily by the laser repetition rate, and sPA image acquisition can take seconds to minutes, depending on the system and imaging volume. We have developed an algorithm that can be used to eliminate extraneous wavelengths and decrease image acquisition time while maintaining image quality. Here, we show the effect the wavelength selection has on in vivo sPA imaging. As an example, dye draining to the lymph node of a mouse was imaged. We demonstrate that sPA image quality is more accurately preserved when wavelengths are chosen based on the spectral features of the absorbers compared to often-used selection of evenly spaced wavelengths. In fact, only three wavelengths are needed to accurately spectrally unmix the dye from oxygenated and deoxygenated hemoglobin.

© 2014 Optical Society of America

OCIS Codes
(170.5120) Medical optics and biotechnology : Photoacoustic imaging
(170.7170) Medical optics and biotechnology : Ultrasound
(300.1030) Spectroscopy : Absorption

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: December 19, 2013
Revised Manuscript: February 11, 2014
Manuscript Accepted: February 22, 2014
Published: March 31, 2014

Virtual Issues
Vol. 9, Iss. 6 Virtual Journal for Biomedical Optics

Geoffrey P. Luke and Stanislav Y. Emelianov, "Optimization of in vivo spectroscopic photoacoustic imaging by smart optical wavelength selection," Opt. Lett. 39, 2214-2217 (2014)

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