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Optics Letters

Optics Letters


  • Editor: Xi-Cheng Zhang
  • Vol. 39, Iss. 8 — Apr. 15, 2014
  • pp: 2262–2265

Real-time quantitative phase reconstruction in off-axis digital holography using multiplexing

Pinhas Girshovitz and Natan T. Shaked  »View Author Affiliations

Optics Letters, Vol. 39, Issue 8, pp. 2262-2265 (2014)

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We present a new approach for obtaining significant speedup in the digital processing of extracting unwrapped phase profiles from off-axis digital holograms. The new technique digitally multiplexes two orthogonal off-axis holograms, where the digital reconstruction, including spatial filtering and two-dimensional phase unwrapping on a decreased number of pixels, can be performed on both holograms together, without redundant operations. Using this technique, we were able to reconstruct, for the first time to our knowledge, unwrapped phase profiles from off-axis holograms with 1 megapixel in more than 30 frames per second using a standard single-core personal computer on a MATLAB platform, without using graphic-processing-unit programming or parallel computing. This new technique is important for real-time quantitative visualization and measurements of highly dynamic samples and is applicable for a wide range of applications, including rapid biological cell imaging and real-time nondestructive testing. After comparing the speedups obtained by the new technique for holograms of various sizes, we present experimental results of real-time quantitative phase visualization of cells flowing rapidly through a microchannel.

© 2014 Optical Society of America

OCIS Codes
(090.4220) Holography : Multiplex holography
(100.5070) Image processing : Phase retrieval
(120.3180) Instrumentation, measurement, and metrology : Interferometry
(090.1995) Holography : Digital holography
(110.3175) Imaging systems : Interferometric imaging
(100.5088) Image processing : Phase unwrapping

ToC Category:
Image Processing

Original Manuscript: January 27, 2014
Revised Manuscript: February 28, 2014
Manuscript Accepted: March 4, 2014
Published: April 8, 2014

Virtual Issues
Vol. 9, Iss. 6 Virtual Journal for Biomedical Optics

Pinhas Girshovitz and Natan T. Shaked, "Real-time quantitative phase reconstruction in off-axis digital holography using multiplexing," Opt. Lett. 39, 2262-2265 (2014)

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