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Optics Letters

Optics Letters


  • Editor: Xi-Cheng Zhang
  • Vol. 39, Iss. 8 — Apr. 15, 2014
  • pp: 2431–2434

Development of a doubly weighted Gerchberg–Saxton algorithm for use in multibeam imaging applications

Simon P. Poland, Nikola Krstajić, Robert D. Knight, Robert K. Henderson, and Simon M. Ameer-Beg  »View Author Affiliations

Optics Letters, Vol. 39, Issue 8, pp. 2431-2434 (2014)

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We report on the development of a doubly weighted Gerchberg–Saxton algorithm (DWGS) to enable generation of uniform beamlet arrays with a spatial light modulator (SLM) for use in multiphoton multifocal imaging applications. The algorithm incorporates the WGS algorithm as well as feedback of fluorescence signals from the sample measured with a single-photon avalanche diode (SPAD) detector array. This technique compensates for issues associated with nonuniform illumination onto the SLM, the effects due to aberrations and the variability in gain between detectors within the SPAD array to generate a uniformly illuminated multiphoton fluorescence image. We demonstrate the use of the DWGS with a number of beamlet array patterns to image muscle fibers of a 5-day-old fixed zebrafish larvae.

© 2014 Optical Society of America

OCIS Codes
(040.1240) Detectors : Arrays
(110.4190) Imaging systems : Multiple imaging
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(180.0180) Microscopy : Microscopy
(230.6120) Optical devices : Spatial light modulators
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Imaging Systems

Original Manuscript: January 29, 2014
Revised Manuscript: February 25, 2014
Manuscript Accepted: February 25, 2014
Published: April 10, 2014

Virtual Issues
Vol. 9, Iss. 6 Virtual Journal for Biomedical Optics

Simon P. Poland, Nikola Krstajić, Robert D. Knight, Robert K. Henderson, and Simon M. Ameer-Beg, "Development of a doubly weighted Gerchberg–Saxton algorithm for use in multibeam imaging applications," Opt. Lett. 39, 2431-2434 (2014)

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