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Optics Letters

Optics Letters


  • Editor: Xi-Cheng Zhang
  • Vol. 39, Iss. 8 — Apr. 15, 2014
  • pp: 2471–2474

Common-path configuration in total internal reflection digital holography microscopy

Alejandro Calabuig, Marcella Matrecano, Melania Paturzo, and Pietro Ferraro  »View Author Affiliations

Optics Letters, Vol. 39, Issue 8, pp. 2471-2474 (2014)

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Total Internal Reflection Digital Holographic Microscopy (TIRDHM) is recognized to be a powerful tool for retrieving quantitative phase images of cell–substrate interfaces, adhesions, and tissue structures close to the prism surface. In this Letter, we develop an improved TIRDHM system, taking advantage of a refractive index mismatch between the prism and the sample substrate, to allow phase-shifting DH with just a single-beam interferometric configuration. Instead of the traditional off-axis method, phase-shift method is used to retrieve amplitude and phase images in coherent light and TIR modality. Essentially, the substrate–prism interface acts like a beam splitter generating a reference beam, where the phase-shift dependence on the incident angle is exploited in this common-path configuration. With the aim to demonstrate the technique’s validity, some experiments are performed to establish the advantage of this compact and simple configuration, in which the reference arm in the setup is avoided.

© 2014 Optical Society of America

OCIS Codes
(050.5080) Diffraction and gratings : Phase shift
(180.0180) Microscopy : Microscopy
(180.3170) Microscopy : Interference microscopy
(260.6970) Physical optics : Total internal reflection
(090.1995) Holography : Digital holography

ToC Category:

Original Manuscript: December 19, 2013
Revised Manuscript: February 28, 2014
Manuscript Accepted: March 17, 2014
Published: April 14, 2014

Virtual Issues
Vol. 9, Iss. 6 Virtual Journal for Biomedical Optics

Alejandro Calabuig, Marcella Matrecano, Melania Paturzo, and Pietro Ferraro, "Common-path configuration in total internal reflection digital holography microscopy," Opt. Lett. 39, 2471-2474 (2014)

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