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Optics Letters

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  • Editor: Xi-Cheng Zhang
  • Vol. 39, Iss. 9 — May. 1, 2014
  • pp: 2569–2572

Automatic method for focusing biological specimens in digital lensless holographic microscopy

Carlos A. Trujillo and Jorge Garcia-Sucerquia  »View Author Affiliations


Optics Letters, Vol. 39, Issue 9, pp. 2569-2572 (2014)
http://dx.doi.org/10.1364/OL.39.002569


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Abstract

A self-focusing method applicable to digital lensless holographic microscopy is presented. The method searches for the global minimum of the area enclosing a given amount of energy in a region surrounding the object of interest. The proposed modified enclosed energy method has been tested on self-focusing experimental holograms of a paramecium specimen and a section of the head of a drosophila melanogaster fly. The presented self-focusing technique also has been contrasted with some of the already reported methods to seek the best focus image.

© 2014 Optical Society of America

OCIS Codes
(180.6900) Microscopy : Three-dimensional microscopy
(260.5950) Physical optics : Self-focusing
(090.1995) Holography : Digital holography

ToC Category:
Microscopy

History
Original Manuscript: February 21, 2014
Revised Manuscript: March 24, 2014
Manuscript Accepted: March 25, 2014
Published: April 18, 2014

Virtual Issues
Vol. 9, Iss. 7 Virtual Journal for Biomedical Optics

Citation
Carlos A. Trujillo and Jorge Garcia-Sucerquia, "Automatic method for focusing biological specimens in digital lensless holographic microscopy," Opt. Lett. 39, 2569-2572 (2014)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-39-9-2569


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