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Optics Letters

Optics Letters


  • Editor: Xi-Cheng Zhang
  • Vol. 39, Iss. 9 — May. 1, 2014
  • pp: 2759–2762

Holographic tracking of living cells by three-dimensional reconstructed complex wavefronts alignment

Pasquale Memmolo, Lisa Miccio, Andrea Finizio, Paolo A. Netti, and Pietro Ferraro  »View Author Affiliations

Optics Letters, Vol. 39, Issue 9, pp. 2759-2762 (2014)

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We propose here a new three-dimensional (3D) holographic tracking method capable to track, simultaneously and in a single step, all the spatial coordinates of micro-objects. The approach is based on the enhanced correlation coefficient (ECC) maximization method but applied, for the first time to the best of our knowledge, directly on the holographic reconstructed complex wave fields. The key novelty of the proposed strategy is its ability to calculate simultaneously the 3D coordinates of cells, without decoupling the contribution of amplitude and phase. The proposed strategy is tested on living cells (i.e., NIH 3T3 mouse fibroblast) flowing into a microfluidic channel and compared with classical holographic tracking approach. Theoretical description and experimental validation of the proposed strategy are reported.

© 2014 Optical Society of America

OCIS Codes
(100.2000) Image processing : Digital image processing
(180.6900) Microscopy : Three-dimensional microscopy
(090.1995) Holography : Digital holography
(100.4999) Image processing : Pattern recognition, target tracking

ToC Category:
Image Processing

Original Manuscript: February 28, 2014
Revised Manuscript: March 26, 2014
Manuscript Accepted: March 28, 2014
Published: April 30, 2014

Virtual Issues
Vol. 9, Iss. 7 Virtual Journal for Biomedical Optics

Pasquale Memmolo, Lisa Miccio, Andrea Finizio, Paolo A. Netti, and Pietro Ferraro, "Holographic tracking of living cells by three-dimensional reconstructed complex wavefronts alignment," Opt. Lett. 39, 2759-2762 (2014)

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