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Novel Techniques in Microscopy (2011)

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  • Novel Techniques in Microscopy (NTM) 2011 paper: JTuA1
  • OSA Technical Digest (CD)
  • Chen, Ying-Ling A; Shi, Lei; Lewis, Jim; Wang, Ming; Vida, Ryan
  • We incorporated anterior chamber components, axial length, and wavefront measurements to construct pilot customized eye models for extensive applications. 21 normal and diseased eyes...

  • Novel Techniques in Microscopy (NTM) 2011 paper: JTuA10
  • OSA Technical Digest (CD)
  • Thompson, Deanna L; McNerney, Gregory; Dale, Benjamin M; Chen, Benjamin K; Huser, Thomas
  • Live, 3D, multicolor imaging of cell-to-cell HIV-1 transmission using spinning disk confocal microscopy and a replication-competent fluorescent clone of the virus reveals clues to...

  • Novel Techniques in Microscopy (NTM) 2011 paper: JTuA11
  • OSA Technical Digest (CD)
  • Small, Alexander R; Yirga, Nahom
  • Gaussian Mask is an algorithm for localizing fluorophores in microscopy. Using simulated images, substantial speed improvements are shown to be possible if good initial position...

  • Novel Techniques in Microscopy (NTM) 2011 paper: JTuA12
  • OSA Technical Digest (CD)
  • Szipocs, Robert; Antal, Peter Gyula; Szigligeti, Attila; Kolonics, Attila
  • We report on a broadly tunable, long-cavity, low-pump-threshold, pulsed Ti:Sapphire laser. The laser delivers nearly transform limited ~300 fs, ~10 nJ pulses at 22 MHz repetition rate...

  • Novel Techniques in Microscopy (NTM) 2011 paper: JTuA13
  • OSA Technical Digest (CD)
  • Pavillon, Nicolas; Kühn, Jonas; Jourdain, Pascal; Depeursinge, Christian D; Magistretti, Pierre J; Marquet, Pierre
  • We demonstrate the capability of digital holography to dynamically detect non-invasively cell death through the measurement of cellular volume regulation, considered as an early...

  • Novel Techniques in Microscopy (NTM) 2011 paper: JTuA14
  • OSA Technical Digest (CD)
  • Binding, Jonas; Benarous, Juliette; Gigan, Sylvain; Boccara, Claude; Léger, Jean-François; Bourdieu, Laurent
  • We demonstrate the ability of full-field OCT to image the cortex of living rats. The main feature that appears is individual myelin fibers. A precise measurement of the brain...

  • Novel Techniques in Microscopy (NTM) 2011 paper: JTuA15
  • OSA Technical Digest (CD)
  • Maitland, Kristen C; Saldua, Meagan; Olsovsky, Cory
  • We exploit a fast motorized translation stage to replace the frame scan mirror in a raster scanning confocal microscope to extend field of view in one axis. 5cm x 1mm image is captured...

  • Novel Techniques in Microscopy (NTM) 2011 paper: JTuA16
  • OSA Technical Digest (CD)
  • Kolodziejski, Noah; Gurjar, Rajan; Wolf, David
  • We have adapted Tip-Enhanced Raman Spectroscopy (TERS) technology to a DNA sequencing modality simultaneously sensitive to a broad spectrum of cancer-relevant lesions. Obstacles...

  • Novel Techniques in Microscopy (NTM) 2011 paper: JTuA17
  • OSA Technical Digest (CD)
  • Yanez, Ciceron; Andrade, Carolina D; Morales, Alma R; Urakami, Takeo; Komatsu, Masanobu; Belfield, Kevin D
  • Efficient two-photon (2PA) absorbing dyes and bioconjugates were used in two-photon fluorescence microscopy (2PFM) of cells, tissue sections, and excised tumors. Results show the...

  • Novel Techniques in Microscopy (NTM) 2011 paper: JTuA18
  • OSA Technical Digest (CD)
  • Appaix, Florence; Roemer, Johannes; van der Sanden, Boudewijn; Girod, Sabine; Boisseau, Sylvie; Albrieux, Mireille; Wege, Hartmut; Guillemain, Isabelle; Depaulis, Antoine; Vial, Jean-Claude A
  • As compared to local injections of sulforhodamine-B and sulforhodamine-101, i.v. administration of these dyes was shown to be more efficient and less invasive for astrocyte staining in...

  • Novel Techniques in Microscopy (NTM) 2011 paper: JTuA19
  • OSA Technical Digest (CD)
  • Campbell, Paul
  • This work aimed to produce monodisperse microbubbles for use as theranostic agents in medical ultrasound. We describe our design for a glass microfluidic chip with a distinctive flow...

  • Novel Techniques in Microscopy (NTM) 2011 paper: JTuA2
  • OSA Technical Digest (CD)
  • Chen, George; Loo, Joachim
  • Phase Shifting Interferometer using the Carre and Hariharan algorithms is proposed for quantifying resorption in bone sample. Advantages of the system include being non-contact, 3D...

  • Novel Techniques in Microscopy (NTM) 2011 paper: JTuA20
  • OSA Technical Digest (CD)
  • Campbell, Paul
  • The turbidity of solutions containing 2 ultrasound contrast agents (SonoVue®,Bracco Diagnostics, Inc. and Sonazoid™, GE HealthCare) was measured as a function of ultrasound...

  • Novel Techniques in Microscopy (NTM) 2011 paper: JTuA21
  • OSA Technical Digest (CD)
  • Frazer, Andrew; Wang, Xuhua; Nguyen, Dao M; Morales, Alma R; Belfield, Kevin D
  • Abstract: We report the synthesis, photophysical characteristics of two photon fluorescent (2PF) probes which shows superior specificity for zinc coupled with two photon microscopy...

  • Novel Techniques in Microscopy (NTM) 2011 paper: JTuA22
  • OSA Technical Digest (CD)
  • Bagchi, Srijeeta; Roy, Debasish; Vasu, Ram Mohan
  • This paper attempts to model the forward problem in photoacoustic tomography (PAT) using discontinuous Galerkin (DG) method. Numerical experiments show that DG solutions are comparable...

  • Novel Techniques in Microscopy (NTM) 2011 paper: JTuA23
  • OSA Technical Digest (CD)
  • Siler, Martin; Karasek, Vitezslav; Zemanek, Pavel
  • Optical forces acting upon a microparticle placed near the surface are evaluated using full 3D solution of Maxwell equations based on the Finite Element Method. The stress is put on...

  • Novel Techniques in Microscopy (NTM) 2011 paper: JTuA24
  • OSA Technical Digest (CD)
  • Sergides, Marios; Skelton, Susan E; Patel, Radhika; Pawlikowska, Agata; Jones, Phil
  • We investigate experimentally and theoretically the trapping of micro- and nanoparticles in the evanescent field surrounding a tapered optical fiber. and show how combinations of modes...

  • Novel Techniques in Microscopy (NTM) 2011 paper: JTuA25
  • OSA Technical Digest (CD)
  • Marago, Onofrio; Jones, Phil; Saija, Rosalba; Borghese, Ferdinando; Denti, Paolo; Iati, Maria A; Gucciardi, Pietro
  • We investigate plasmon-enhanced trapping of metal nanoparticles. We calculate the optical forces on gold, silver and aluminium nanospheres through a procedure based on the Maxwell...

  • Novel Techniques in Microscopy (NTM) 2011 paper: JTuA26
  • OSA Technical Digest (CD)
  • Skelton, Susan E; Sergides, Marios; Patel, Radhika; Pawlikowska, Agata; Marago, Onofrio; Jones, Phil
  • We present experimental measurements of the spring constants of a radially polarized optical tweezer for a wide range of micro- and nano-particles and compare the results to those...

  • Novel Techniques in Microscopy (NTM) 2011 paper: JTuA27
  • OSA Technical Digest (CD)
  • Campbell, Paul
  • Cavitation science has experienced heightened interest within medical contexts due to the emerging theranostic capabilities of ultrasound driven microbubbles. We review the state of...
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