Optics InfoBase: Spotlight on Optics - Flow visualization and flow cytometry with holographic video microscopy

Spotlight on Optics

| HIGHLIGHTED ARTICLES FROM OSA JOURNALS

July 2009

Flow visualization and flow cytometry with holographic video microscopy

Published in Optics Express, Vol. 17 Issue 15, pp.13071-13079 (2009)
by Fook Chiong Cheong, Bo Sun, Rémi Dreyfus, Jesse Amato-Grill, Ke Xiao, Lisa Dixon, and David G. Grier

Source article Abstract | Full Text: XHTML | Full Text: PDF

Spotlight summary: A pleasurable read! A flow cytometry system is presented in a well-written paper that contains a solid analysis and fine results. The size and the refractive index of individual colloidal particles are accurately measured, and their three-dimensional motion is tracked in near real time from the video stream captured by an in-line holographic microscope—without the need of staining of fluorescent labeling. The multimedia content, although not mentioned specifically in the paper, is especially appropriate.

Technical Division: Optical Design and Instrumentation
ToC Category: Instrumentation, Measurement, and Metrology

OCIS Codes:	(100.2960) Image processing : Image analysis
	(120.7250) Instrumentation, measurement, and metrology : Velocimetry
	(180.6900) Microscopy : Three-dimensional microscopy
	(090.1995) Holography : Digital holography

Posted on July 17, 2009