OSA's Digital Library

Spotlight on Optics

Spotlight on Optics

| HIGHLIGHTED ARTICLES FROM OSA JOURNALS

  • February 2010

Optics InfoBase > Spotlight on Optics > Realistic modeling of the illumination point spread function in confocal scanning optical microscopy


Realistic modeling of the illumination point spread function in confocal scanning optical microscopy

Published in JOSA A, Vol. 27 Issue 2, pp.295-302 (2010)
by Michael J. Nasse and Jörg C. Woehl

Source article Abstract | Full Text: XHTML | Full Text: PDF


Spotlight summary: The authors present a fully vectorial theory for evaluating the point spread function of the illumination in a confocal microscope. The illuminating beam is assumed to pass through stratified media before reaching the sample. A program implementing the theory was produced by the authors and can be found on the net for free (http://onemolecule.chem.uwm.edu/software). This program allows the interested readers to study in any detail the field in the focal region, with the help of efficient color pictures, and should be of considerable help for scientists working with confocal microscopy.

Technical Division: Optics in Biology and Medicine
ToC Category: Microscopy
OCIS Codes: (050.1960) Diffraction and gratings : Diffraction theory
(180.0180) Microscopy : Microscopy
(180.1790) Microscopy : Confocal microscopy
(260.0260) Physical optics : Physical optics


Posted on February 11, 2010

Add Comment
You must log in to add comments.





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Previous Spotlights

2014

2013

2012

2011

2010

2009