The reconstruction of internal light sources in bioluminescence tomography (BLT) is a challenging inverse problem because of the limited amount of information available compared with that for other kinds of tomography such as fluorescence tomography in which external illumination sources are used. We demonstrated previously, using phantom experiments, that a target containing luciferases could be detected tomographically when the target was located relatively close to the imaging boundary. Here we describe an improved BLT reconstruction method that can detect luciferase-containing targets located anywhere within an imaging domain. The method is tested with numerical simulations and further confirmed with several phantom experiments.
© 2006 Optical Society of America
Original Manuscript: August 22, 2005
Revised Manuscript: November 22, 2005
Manuscript Accepted: November 28, 2005
Vol. 1, Iss. 6 Virtual Journal for Biomedical Optics
Senhu Li, Qizhi Zhang, and Huabei Jiang, "Two-dimensional bioluminescence tomography: numerical simulations and phantom experiments," Appl. Opt. 45, 3390-3394 (2006)